RGD Reference Report - Thyroid hormone stimulates myoglobin gene expression in rat cardiac muscle. - Rat Genome Database

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Thyroid hormone stimulates myoglobin gene expression in rat cardiac muscle.

Authors: Giannocco, G  DosSantos, RA  Nunes, MT 
Citation: Giannocco G, etal., Mol Cell Endocrinol. 2004 Oct 29;226(1-2):19-26.
RGD ID: 1582397
Pubmed: PMID:15489001   (View Abstract at PubMed)
DOI: DOI:10.1016/j.mce.2004.07.007   (Journal Full-text)

T3 increases the heart activity, O2 consumption and the reactive O2 species production. Myoglobin (Mb) is highly expressed in the heart, where it facilitates O2 diffusion, mitochondrial respiration, and scavenges reactive O2 species. Here we investigate, by dose-response (0.3-100 microg/100 g BW, i.p., 5 days) and time-course studies (100 microg/100 g BW, i.v., from 0.5 to 24h), whether T3 affects the Mb mRNA and protein expression in atrium (A) and ventricle (V), by Northern and Western blot. We show that the Mb gene is controlled by T3 in A and V, as indicated by Mb mRNA and protein content decrease in thyroidectomized (Tx) rats, and restoration by T3 treatment. In the A, the different doses of T3 induced the Mb mRNA and protein recovery to the euthyroid levels; in the time-course study, this occurred only with the protein levels. In the V, T3 progressively increased the Mb mRNA above the euthyroid levels at a dose of 25 microg/100g BW; higher doses decreased it to the euthyroid levels. Mb protein increased only to the euthyroid levels at all T3 doses injected. The time-course study showed a progressive increase in the ventricular Mb mRNA and protein, which exceeded the euthyroid levels from 6 to 24h, and at 2 and 6 h of the T3 treatment, respectively. We conclude that heart Mb gene expression is influenced by thyroid status.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MbRatresponse to hormone  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mb  (myoglobin)

Objects referenced in this article
Gene MMP7 matrix metallopeptidase 7 Homo sapiens
Gene Mmp7 matrix metallopeptidase 7 Mus musculus
Gene Mmp7 matrix metallopeptidase 7 Rattus norvegicus

Additional Information