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CD2AP contributes to cell migration and adhesion in cultured gastric epithelium.

Authors: Mustonen, H  Lepisto, A  Lehtonen, S  Lehtonen, E  Puolakkainen, P  Kivilaakso, E 
Citation: Mustonen H, etal., Biochem Biophys Res Commun. 2005 Jul 1;332(2):426-32.
Pubmed: (View Article at PubMed) PMID:15910750
DOI: Full-text: DOI:10.1016/j.bbrc.2005.04.140

The potential association of CD2AP with the adherens junction protein E-cadherin, co-localization with the actin cytoskeleton, and involvement in cell migration was investigated in cultured rat gastric mucosal cells. In stationary cells, CD2AP was localized perinuclearly while E-cadherin was expressed along cell-cell contacts and F-actin formed a branched network and adhesion belts. In migrating cells, CD2AP appeared as thread-like accumulations in the leading edges, colocalizing with F-actin and occasionally with E-cadherin. Intracellular injection of anti-CD2AP significantly retarded the migration speed of the cells suggesting a crucial role for CD2AP in mucosal cell migration, possibly as a scaffolding protein between cell membrane proteins and actin cytoskeleton. Co-immunoprecipitation assays revealed that CD2AP and E-cadherin are in a complex in HGF stimulated cells. It is concluded that CD2AP interacts with E-cadherin and co-localizes with F-actin in the leading edge of migrating cells, and significantly contributes to cell migration in restituting gastric epithelium.


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RGD Object Information
RGD ID: 1582380
Created: 2006-11-07
Species: All species
Last Modified: 2006-11-07
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.