RGD Reference Report - The interaction of rat liver carbamoyl phosphate synthetase and ornithine transcarbamoylase with inner mitochondrial membranes. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

The interaction of rat liver carbamoyl phosphate synthetase and ornithine transcarbamoylase with inner mitochondrial membranes.

Authors: Powers-Lee, SG  Mastico, RA  Bendayan, M 
Citation: Powers-Lee SG, etal., J Biol Chem. 1987 Nov 15;262(32):15683-8.
RGD ID: 1582379
Pubmed: PMID:3680220   (View Abstract at PubMed)

The intramitochondrial localization of the urea cycle enzymes, carbamoyl phosphate synthetase and ornithine transcarbamoylase, has been examined by both in vitro and in situ studies. The following three lines of evidence are presented to establish that significant fractions of the rat liver enzymes are loosely associated with the inner mitochondrial membrane: 1) when the mitochondrion is fractionated, the enzymes partition between the matrix and membrane fractions in the absence of detergent and partition solely to the matrix in the presence of detergent; 2) the purified enzymes associate with purified inner membrane preparations; and, 3) protein A-gold electron microscopic immunocytochemical analysis of rat liver sections reveals a nonrandom arrangement of the enzyme, with the maximal enzyme density adjacent to the inner mitochondrial membrane. These findings serve as the basis for novel potential mechanisms for regulation of the activity of the enzymes and provide additional evidence for the extensive organization of the mitochondrial matrix. The membrane interaction might also serve as the organizing factor for a carbamoyl phosphate synthetase-ornithine transcarbamoylase or other multienzyme complex.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
urea cycle  IDA 1582379; 1582379 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
mitochondrial inner membrane  IDA 1582379; 1582379 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
carbamoyl-phosphate synthase (ammonia) activity  IDA 1582379 RGD 
ornithine carbamoyltransferase activity  IDA 1582379 RGD 
phospholipid binding  IDA 1582379; 1582379 RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
urea cycle pathway   ISOCps1 (Rattus norvegicus)1582379; 1582379 RGD 
urea cycle pathway   IDA 1582379; 1582379 RGD 
urea cycle pathway   ISOOtc (Rattus norvegicus)1582379; 1582379 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Cps1  (carbamoyl-phosphate synthase 1)
Otc  (ornithine transcarbamylase)

Genes (Mus musculus)
Cps1  (carbamoyl-phosphate synthetase 1)
Otc  (ornithine transcarbamylase)

Genes (Homo sapiens)
CPS1  (carbamoyl-phosphate synthase 1)
OTC  (ornithine transcarbamylase)


Additional Information