RGD Reference Report - Caveolin regulates microtubule polymerization in the vascular smooth muscle cells. - Rat Genome Database

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Caveolin regulates microtubule polymerization in the vascular smooth muscle cells.

Authors: Kawabe, J  Okumura, S  Nathanson, MA  Hasebe, N  Ishikawa, Y 
Citation: Kawabe J, etal., Biochem Biophys Res Commun. 2006 Mar 31;342(1):164-9. Epub 2006 Feb 3.
RGD ID: 1582162
Pubmed: (View Article at PubMed) PMID:16480946
DOI: Full-text: DOI:10.1016/j.bbrc.2006.01.125

Microtubule and caveolin have common properties in intracellular trafficking and the regulation of cellular growth. Overexpression of caveolin in vascular smooth muscle cells increased the polymer form of microtubule without changing in the total amount of tubulin, and downregulation of caveolin decreased the polymer form of microtubule. Fractionation of cellular proteins followed by immunodetection as well as immunostaining of caveolin and microtubule revealed that caveolin and a portion of microtubule were co-localized in caveolar fractions. A caveolin scaffolding domain peptide, which mimics caveolin function, did not alter the polymerization of microtubule in vitro, but dramatically inhibited the depolymerization of microtubule induced by stathmin, a microtubule destabilizing protein, which was also found in caveolar fractions. Accordingly, it is most likely that caveolin increased the polymer form of microtubule through the inhibition of a microtubule destabilizer, stathmin, suggesting a novel role of caveolin in regulating cellular network and trafficking.

Annotation

Gene Ontology Annotations    

Biological Process

Cellular Component
caveola  (IDA)

Objects Annotated

Genes (Rattus norvegicus)
Cav1  (caveolin 1)
Cav3  (caveolin 3)


Additional Information