RGD Reference Report - Epidermal growth factor receptor distribution during chemotactic responses. - Rat Genome Database

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Epidermal growth factor receptor distribution during chemotactic responses.

Authors: Bailly, M  Wyckoff, J  Bouzahzah, B  Hammerman, R  Sylvestre, V  Cammer, M  Pestell, R  Segall, JE 
Citation: Bailly M, etal., Mol Biol Cell. 2000 Nov;11(11):3873-83.
RGD ID: 1581916
Pubmed: PMID:11071913   (View Abstract at PubMed)
PMCID: PMC15043   (View Article at PubMed Central)

To determine the distribution of the epidermal growth factor (EGF) receptor (EGFR) on the surface of cells responding to EGF as a chemoattractant, an EGFR-green fluorescent protein chimera was expressed in the MTLn3 mammary carcinoma cell line. The chimera was functional and easily visualized on the cell surface. In contrast to other studies indicating that the EGFR might be localized to certain regions of the plasma membrane, we found that the chimera is homogeneously distributed on the plasma membrane and becomes most concentrated in vesicles after endocytosis. In spatial gradients of EGF, endocytosed receptor accumulates on the upgradient side of the cell. Visualization of the binding of fluorescent EGF to cells reveals that the affinity properties of the receptor, together with its expression level on cells, can provide an initial amplification step in spatial gradient sensing.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
endocytic vesicle  IDA 1581916 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Egfr  (epidermal growth factor receptor)


Additional Information