RGD Reference Report - Substitution Mapping in Dahl Rats Identifies Two Distinct Blood Pressure Quantitative Trait Loci within 1.12 Mb and 1.25 Mb Intervals on Chromosome 3. - Rat Genome Database

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Substitution Mapping in Dahl Rats Identifies Two Distinct Blood Pressure Quantitative Trait Loci within 1.12 Mb and 1.25 Mb Intervals on Chromosome 3.

Authors: Lee, SJ  Liu, J  Westcott, AM  Vieth, JA  Deraedt, SJ  Yang, S  Joe, B  Cicila, GT 
Citation: Lee SJ, etal., Genetics. 2006 Oct 8;.
RGD ID: 1581650
Pubmed: (View Article at PubMed) PMID:17028336
DOI: Full-text: DOI:10.1534/genetics.106.061747

Substitution mapping was used to refine the localization of blood pressure (BP) quantitative trait loci (QTLs) within the congenic region of S.R-Edn3 rats located at the q-terminus of rat chromosome 3 (RNO3). An F2(SxS.R-Edn3) population (n=173) was screened to identify rats having crossovers within the congenic region of RNO3 and six congenic substrains were developed that carry shorter segments of R-rat derived RNO3. Five of the six congenic substrains had significantly lower BP compared to the parental S rat. The lack of BP lowering effect demonstrated by the S.R(ET3x5) substrain and the BP lowering effect retained by S.R(ET3x2) substrain, together define the RNO3 BP QTL-containing region as approximately 4.64 Mb. Two non-overlapping substrains, S.R(ET3x1) and S.R(ET3x6), had significantly lower BP compared to the S strain, indicating the presence of two distinct BP QTLs in the RNO3 q-terminus. The RNO3 q-terminus was fine-mapped with newly developed polymorphic markers to characterize the extent of the congenic regions. The two RNO3 BP QTL regions were thus defined as within intervals of 0.05-1.12 Mb and 0.72-1.25 Mb, respectively. Also important was our difficulty in fine-mapping and marker placement in this portion of the rat genome (and thus candidate gene identification) using the available genomic data, including the rat genome sequence.



Disease Annotations    
Hypotension  (IDA)

Phenotype Annotations    

Mammalian Phenotype

Objects Annotated

QTLs
Bp285  (Blood pressure QTL 285)
Bp286  (Blood pressure QTL 286)

Objects referenced in this article
Marker D3Mco35 D3Mco35 Rattus norvegicus
Marker D3Mco71 D3Mco71 Rattus norvegicus
Marker D3Mco41 D3Mco41 Rattus norvegicus
Marker D3Mco85 D3Mco85 Rattus norvegicus
Marker D3Mco67 D3Mco67 Rattus norvegicus
Marker D3Mco84 D3Mco84 Rattus norvegicus
Marker D3Mco36 D3Mco36 Rattus norvegicus
Marker D3Mco54 D3Mco54 Rattus norvegicus
Marker D3Mco79 D3Mco79 Rattus norvegicus
Marker D3Mco83 D3Mco83 Rattus norvegicus
Marker D3Mco66 D3Mco66 Rattus norvegicus
Marker D3Mco52 D3Mco52 Rattus norvegicus
Marker D3Mco40 D3Mco40 Rattus norvegicus
Marker D3Mco59 D3Mco59 Rattus norvegicus
Marker D3Mco44 D3Mco44 Rattus norvegicus
Marker D3Mco58 D3Mco58 Rattus norvegicus
Marker D3Mco65 D3Mco65 Rattus norvegicus
Marker D3Mco61 D3Mco61 Rattus norvegicus
Marker D3Mco49 D3Mco49 Rattus norvegicus
Marker D3Mco69 D3Mco69 Rattus norvegicus
Marker D3Mco51 D3Mco51 Rattus norvegicus
Marker D3Mco37 D3Mco37 Rattus norvegicus
Marker D3Mco76 D3Mco76 Rattus norvegicus
Marker D3Mco48 D3Mco48 Rattus norvegicus
Marker D3Mco55 D3Mco55 Rattus norvegicus
Marker D3Mco39 D3Mco39 Rattus norvegicus
Marker D3Mco78 D3Mco78 Rattus norvegicus
Marker D3Mco45 D3Mco45 Rattus norvegicus
Marker D3Mco80 D3Mco80 Rattus norvegicus
Marker D3Mco56 D3Mco56 Rattus norvegicus
Marker D3Mco38 D3Mco38 Rattus norvegicus
Marker D3Mco64 D3Mco64 Rattus norvegicus
Marker D3Mco81 D3Mco81 Rattus norvegicus
Marker D3Mco47 D3Mco47 Rattus norvegicus
Marker D3Mco62 D3Mco62 Rattus norvegicus
Marker D3Mco43 D3Mco43 Rattus norvegicus
Marker D3Mco82 D3Mco82 Rattus norvegicus
Marker D3Mco50 D3Mco50 Rattus norvegicus
Marker D3Mco73 D3Mco73 Rattus norvegicus
Marker D3Mco70 D3Mco70 Rattus norvegicus
Marker D3Mco34 D3Mco34 Rattus norvegicus
Marker D3Mco57 D3Mco57 Rattus norvegicus
Marker D3Mco74 D3Mco74 Rattus norvegicus
Marker D3Mco60 D3Mco60 Rattus norvegicus
Marker D3Mco68 D3Mco68 Rattus norvegicus
Marker D3Mco53 D3Mco53 Rattus norvegicus
Marker D3Mco46 D3Mco46 Rattus norvegicus
Marker D3Mco77 D3Mco77 Rattus norvegicus
Marker D3Mco72 D3Mco72 Rattus norvegicus
Marker D3Mco63 D3Mco63 Rattus norvegicus
Marker D3Mco42 D3Mco42 Rattus norvegicus
Marker D3Mco75 D3Mco75 Rattus norvegicus
Strain SS.SR-(D3Arb14-D3Mco36)/Mco null Rattus norvegicus
Strain SS.SR-(D3Mco78-D3Got130)/Jr null Rattus norvegicus

Additional Information