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A role for the spectrin superfamily member Syne-1 and kinesin II in cytokinesis.

Authors: Fan, J  Beck, KA 
Citation: Fan J and Beck KA, J Cell Sci. 2004 Feb 1;117(Pt 4):619-29. Epub 2004 Jan 6.
Pubmed: (View Article at PubMed) PMID:14709720
DOI: Full-text: DOI:10.1242/jcs.00892

Expression of a dominant negative fragment of the spectrin family member Syne-1 causes an accumulation of binucleate cells, suggesting a role for this protein in cytokinesis. An association of this fragment with the C-terminal tail domain of the kinesin II subunit KIF3B was identified by yeast two-hybrid and co-precipitation assays, suggesting that the role of Syne-1 in cytokinesis involves an interaction with kinesin II. In support of this we found that (1) expression of KIF3B tail domain also gives rise to multinucleate cells, (2) both Syne-1 and KIF3B localize to the central spindle and midbody during cytokinesis in a detergent resistant and ATP sensitive manner and (3) Syne-1 localization is blocked by expression of KIF3B tail. Also, membrane vesicles containing syntaxin associate with the spindle midbody with identical properties. We conclude that Syne-1 and KIF3B function together in cytokinesis by facilitating the accumulation of membrane vesicles at the spindle midbody.

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RGD Object Information
RGD ID: 1581598
Created: 2006-10-07
Species: All species
Last Modified: 2006-10-07
Status: ACTIVE



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