RGD Reference Report - Atrial natriuretic factor stimulates exocrine pancreatic secretion in the rat through NPR-C receptors. - Rat Genome Database

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Atrial natriuretic factor stimulates exocrine pancreatic secretion in the rat through NPR-C receptors.

Authors: Sabbatini, ME  Villagra, A  Davio, CA  Vatta, MS  Fernandez, BE  Bianciotti, LG 
Citation: Sabbatini ME, etal., Am J Physiol Gastrointest Liver Physiol. 2003 Nov;285(5):G929-37. Epub 2003 Jun 26.
RGD ID: 1581460
Pubmed: PMID:12829435   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpgi.00010.2003   (Journal Full-text)

Increasing evidence supports the role of atrial natriuretic factor (ANF) in the modulation of gastrointestinal physiology. The effect of ANF on exocrine pancreatic secretion and the possible receptors and pathways involved were studied in vivo. Anesthetized rats were prepared with pancreatic duct cannulation, pyloric ligation, and bile diversion into the duodenum. ANF dose-dependently increased pancreatic secretion of fluid and proteins and enhanced secretin and CCK-evoked response. ANF decreased chloride secretion and increased the pH of the pancreatic juice. Neither cholinergic nor adrenergic blockade affected ANF-stimulated pancreatic secretion. Furthermore, ANF response was not mediated by the release of nitric oxide. ANF-evoked protein secretion was not inhibited by truncal vagotomy, atropine, or Nomega-nitro-l-arginine methyl ester administration. The selective natriuretic peptide receptor-C (NPR-C) receptor agonist cANP-(4-23) mimicked ANF response in a dose-dependent fashion. When the intracellular signaling coupled to NPR-C receptors was investigated in isolated pancreatic acini, results showed that ANF did not modify basal or forskolin-evoked cAMP formation, but it dose-dependently enhanced phosphoinositide hydrolysis, which was blocked by the selective PLC inhibitor U-73122. ANF stimulated exocrine pancreatic secretion in the rat, and its effect was not mediated by nitric oxide or parasympathetic or sympathetic activity. Furthermore, CCK and secretin appear not to be involved in ANF response. Present findings support that ANF exerts a stimulatory effect on pancreatic exocrine secretion mediated by NPR-C receptors coupled to the phosphoinositide pathway.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Npr3Ratpositive regulation of pancreatic juice secretion  IMP  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Objects Annotated

Genes (Rattus norvegicus)
Npr3  (natriuretic peptide receptor 3)

Genes (Mus musculus)
Npr3  (natriuretic peptide receptor 3)

Genes (Homo sapiens)
NPR3  (natriuretic peptide receptor 3)


Additional Information