RGD Reference Report - Kv4 potassium channel subunits control action potential repolarization and frequency-dependent broadening in rat hippocampal CA1 pyramidal neurones. - Rat Genome Database

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Kv4 potassium channel subunits control action potential repolarization and frequency-dependent broadening in rat hippocampal CA1 pyramidal neurones.

Authors: Kim, J  Wei, DS  Hoffman, DA 
Citation: Kim J, etal., J Physiol. 2005 Nov 15;569(Pt 1):41-57. Epub 2005 Sep 1.
RGD ID: 1581430
Pubmed: PMID:16141270   (View Abstract at PubMed)
PMCID: PMC1464206   (View Article at PubMed Central)
DOI: DOI:10.1113/jphysiol.2005.095042   (Journal Full-text)

A-type potassium channels regulate neuronal firing frequency and the back-propagation of action potentials (APs) into dendrites of hippocampal CA1 pyramidal neurones. Recent molecular cloning studies have found several families of voltage-gated K(+) channel genes expressed in the mammalian brain. At present, information regarding the relationship between the protein products of these genes and the various neuronal functions performed by voltage-gated K(+) channels is lacking. Here we used a combination of molecular, electrophysiological and imaging techniques to show that one such gene, Kv4.2, controls AP half-width, frequency-dependent AP broadening and dendritic action potential propagation. Using a modified Sindbis virus, we expressed either the enhanced green fluorescence protein (EGFP)-tagged Kv4.2 or an EGFP-tagged dominant negative mutant of Kv4.2 (Kv4.2g(W362F)) in CA1 pyramidal neurones of organotypic slice cultures. Neurones expressing Kv4.2g(W362F) displayed broader action potentials with an increase in frequency-dependent AP broadening during a train compared with control neurones. In addition, Ca(2)(+) imaging of Kv4.2g(W362F) expressing dendrites revealed enhanced AP back-propagation compared to control neurones. Conversely, neurones expressing an increased A-type current through overexpression of Kv4.2 displayed narrower APs with less frequency dependent broadening and decreased dendritic propagation. These results point to Kv4.2 as the major contributor to the A-current in hippocampal CA1 neurones and suggest a prominent role for Kv4.2 in regulating AP shape and dendritic signalling. As Ca(2)(+) influx occurs primarily during AP repolarization, Kv4.2 activity can regulate cellular processes involving Ca(2)(+)-dependent second messenger cascades such as gene expression and synaptic plasticity.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnd2Rataction potential  IMP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnd2Ratdendritic spine  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Objects Annotated

Genes (Rattus norvegicus)
Kcnd1  (potassium voltage-gated channel subfamily D member 1)
Kcnd2  (potassium voltage-gated channel subfamily D member 2)
Kcnd3  (potassium voltage-gated channel subfamily D member 3)

Genes (Mus musculus)
Kcnd1  (potassium voltage-gated channel, Shal-related family, member 1)
Kcnd2  (potassium voltage-gated channel, Shal-related family, member 2)
Kcnd3  (potassium voltage-gated channel, Shal-related family, member 3)

Genes (Homo sapiens)
KCND1  (potassium voltage-gated channel subfamily D member 1)
KCND2  (potassium voltage-gated channel subfamily D member 2)
KCND3  (potassium voltage-gated channel subfamily D member 3)


Additional Information