RGD Reference Report - Dystrophin Dp71 in PC12 cell adhesion. - Rat Genome Database

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Dystrophin Dp71 in PC12 cell adhesion.

Authors: Enriquez-Aragon, JA  Cerna-Cortes, J  Bermudez de Leon, M  Garcia-Sierra, F  Gonzalez, E  Mornet, D  Cisneros, B 
Citation: Enriquez-Aragon JA, etal., Neuroreport. 2005 Feb 28;16(3):235-8.
RGD ID: 1581349
Pubmed: PMID:15706226   (View Abstract at PubMed)
PMCID: PMC2494584   (View Article at PubMed Central)

Previously, we reported that PC12 cells with decreased Dp71 expression (antisense-Dp71 cells) display deficient nerve-growth-factor-induced neurite outgrowth. In this study, we show that disturbed neurite outgrowth of antisense-Dp71 cells is accompanied by decreased adhesion activity on laminin, collagen and fibronectin. In wild-type cells, the immunostaining of Dp71 and beta1-integrin overlaps in the basal area contacting the substrate, but staining of both proteins decrease in the antisense-Dp71 cells. Morphology of antisense-Dp71 cells at the electron microscopic level is characterized by the lack of filopodia, cellular projections involved in adhesion. Our findings suggest that Dp71 is required for the efficient PC12 cell attachment to beta1-integrin-dependent substrata and that decreased adhesion activity of the antisense-Dp71 cells could determine their deficiency to extend neurites.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
neuron projection morphogenesis  IMP 1581349 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dmd  (dystrophin)


Additional Information