RGD Reference Report - Increased gene expression of antioxidant enzymes in KKAy diabetic mice but not in STZ diabetic mice. - Rat Genome Database

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Increased gene expression of antioxidant enzymes in KKAy diabetic mice but not in STZ diabetic mice.

Authors: Fujita, A  Sasaki, H  Ogawa, K  Okamoto, K  Matsuno, S  Matsumoto, E  Furuta, H  Nishi, M  Nakao, T  Tsuno, T  Taniguchi, H  Nanjo, K 
Citation: Fujita A, etal., Diabetes Res Clin Pract. 2005 Aug;69(2):113-9.
RGD ID: 1581216
Pubmed: PMID:16005359   (View Abstract at PubMed)
DOI: DOI:10.1016/j.diabres.2004.11.016   (Journal Full-text)

Oxidative stress and the gene expression at the transcriptional level of antioxidant enzymes were investigated in two models of diabetes in mice. We used KKAy mice as a model of obese insulin-resistant diabetes, and streptozotocin-induced diabetic mice (STZ mice) as a model of insulin-deficient diabetes. C57BL mice and saline-injected ICR mice were used as the respective non-diabetic controls. To assess oxidative damage, plasma malonedialdehyde (MDA), urine 8-isoprostane and 8-hydroxy deoxyguanosine (8-OHdG) were measured. The mRNA expression of antioxidant enzymes, superoxide dismutase 1 (SOD-1) and glutathione peroxidase 1 (GPx-1) in the kidney and heart were quantified using a real-time polymerase chain reaction. The KKAy mice demonstrated moderate hyperglycemia and hyperlipidemia, and the STZ mice showed severe hyperglycemia and hypolipidemia. The KKAy mice, but not the STZ mice, showed elevated plasma MDA relative to the non-diabetic controls. Urine 8-isoprostane and 8-OHdG in both diabetic mouse groups increased significantly. The urine oxidative stress markers in the severely hyperglycemic STZ mice were higher than those in the moderately hyperglycemic KKAy mice. Although GPx-1 and SOD-1 showed elevated mRNA expression in the KKAy mice in the kidney and heart, in the STZ mice they did not increase compared to the controls. The compensatory up-regulation of the mRNA expression of antioxidant enzymes may be impaired in the insulin-deficient severely hyperglycemic state.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
type 2 diabetes mellitus  ISOSod1 (Mus musculus)1581216; 1581216 RGD 
type 2 diabetes mellitus  IDA 1581216 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Sod1  (superoxide dismutase 1)

Genes (Mus musculus)
Sod1  (superoxide dismutase 1, soluble)

Genes (Homo sapiens)
SOD1  (superoxide dismutase 1)


Additional Information