RGD Reference Report - Expression of the LXRalpha protein in human atherosclerotic lesions. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Expression of the LXRalpha protein in human atherosclerotic lesions.

Authors: Watanabe, Y  Jiang, S  Takabe, W  Ohashi, R  Tanaka, T  Uchiyama, Y  Katsumi, K  Iwanari, H  Noguchi, N  Naito, M  Hamakubo, T  Kodama, T 
Citation: Watanabe Y, etal., Arterioscler Thromb Vasc Biol. 2005 Mar;25(3):622-7. Epub 2004 Dec 29.
RGD ID: 1580788
Pubmed: PMID:15625283   (View Abstract at PubMed)
DOI: DOI:10.1161/01.ATV.0000154489.53077.4e   (Journal Full-text)

OBJECTIVE: Liver X-activated receptor alpha (LXRalpha) regulates multiple genes controlling cholesterol metabolism and transport. To clarify its role in atherogenesis, we established a monoclonal antibody recognizing native human LXRalpha protein and studied the expression pattern in human atherosclerotic lesions. METHODS AND RESULTS: A novel monoclonal antibody PPZ0412 was raised against the ligand-binding domain of LXRalpha, which can be used for immunostaining of human LXRalpha protein. LXRalpha protein was detected in the nucleus of macrophages in the liver, spleen, or lung and also in hepatocytes and adipocytes. In atherosclerotic lesions, the LXRalpha protein was detected in macrophages positive for scavenger receptor class A and/or CD68. CONCLUSIONS: In the human body, the LXRalpha protein is highly expressed in macrophage lineage cells and foam cells in atherosclerotic lesions and is identified as a target for intervention in atherosclerotic disease.

Objects referenced in this article
Gene NR1H3 nuclear receptor subfamily 1 group H member 3 Homo sapiens
Gene Nr1h3 nuclear receptor subfamily 1, group H, member 3 Mus musculus
Gene Nr1h3 nuclear receptor subfamily 1, group H, member 3 Rattus norvegicus

Additional Information