RGD Reference Report - Downregulation of carnitine acyltransferases and organic cation transporter OCTN2 in mononuclear cells in healthy elderly and patients with myelodysplastic syndromes. - Rat Genome Database

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Downregulation of carnitine acyltransferases and organic cation transporter OCTN2 in mononuclear cells in healthy elderly and patients with myelodysplastic syndromes.

Authors: Karlic, H  Lohninger, A  Laschan, C  Lapin, A  Bohmer, F  Huemer, M  Guthann, E  Rappold, E  Pfeilstocker, M 
Citation: Karlic H, etal., J Mol Med. 2003 Jul;81(7):435-42. Epub 2003 Jun 12.
RGD ID: 1580610
Pubmed: PMID:12802501   (View Abstract at PubMed)
DOI: DOI:10.1007/s00109-003-0447-6   (Journal Full-text)

Changes in key enzymes of oxidative metabolism at the mitochondrial level are known to be associated with the aging process, apoptosis, and many diseases. Considering the risk of acquiring a myelodysplastic syndrome (MDS) with age, the aim of this study was to quantify mRNA synthesis of the carnitine palmitoyltransferases (CPT1 and CPT2), carnitine acetyltransferase (CRAT), human specific microsomal CPT, and OCTN2 (organic cation transporter) in mononuclear cells of healthy humans of different age groups and MDS patients. Using quantitative reverse transcriptase real-time PCR we compared mRNA synthesis of the above mentioned enzymes in mononuclear cells from peripheral blood of 23 healthy persons (mean age 45 years), 9 blood and 22 bone marrow samples of 31 MDS patients with varying proportions of apoptotic cells (mean age 78 years), and blood samples of 30 age-matched controls. In addition, plasma carnitine levels were determined. Compared to younger adults, there was a 50% downregulation of CPT1 in elderly persons and in MDS patients. Reduction in CRAT, CPT 2, and OCTN2 was more than 85%. Reduction in microsomal CPT was more pronounced in MDS patients than in age-matched controls (96% vs. 43%). In MDS bone marrow cells there was a negative correlation of CPT1 and CRAT with the relative proportion of apoptotic cells. Plasma carnitine values were similar in all groups. The described reduction in transcription of different genes in blood cells which is well known in different tissues may reflect a systemic signaling process, associated with aging, apoptosis, and MDS.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
myelodysplastic syndrome  IEP 1580610mRNA:decreased expression:PBMCs (human)RGD 
myelodysplastic syndrome  ISOSLC22A5 (Homo sapiens)1580610; 1580610mRNA:decreased expression:PBMCs (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc22a5  (solute carrier family 22 member 5)

Genes (Mus musculus)
Slc22a5  (solute carrier family 22 (organic cation transporter), member 5)

Genes (Homo sapiens)
SLC22A5  (solute carrier family 22 member 5)


Additional Information