RGD Reference Report - Effect of tumor necrosis factor-alpha on developing optic nerve oligodendrocytes in culture. - Rat Genome Database

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Effect of tumor necrosis factor-alpha on developing optic nerve oligodendrocytes in culture.

Authors: Pang, Y  Cai, Z  Rhodes, PG 
Citation: Pang Y, etal., J Neurosci Res. 2005 Apr 15;80(2):226-34.
RGD ID: 1580604
Pubmed: PMID:15765524   (View Abstract at PubMed)
DOI: DOI:10.1002/jnr.20450   (Journal Full-text)

There is increasing evidence that proinflammatory cytokines are involved in the development of periventricular leukomalacia (PVL), a condition in which developing oliodendrocytes (OLs) are preferentially injured. In the present study, we utilized an in vitro assay to demonstrate that the A2B5+ OL progenitors as well as the O4+ prooligodendrocytes (pro-OLs) were more susceptible to tumor necrosis factor-alpha (TNF-alpha) cytotoxicity than the O4+/O1+ immature OLs. OL progenitors were isolated from optic nerves of 7-day-old rat pups and cultured in chemically defined medium supplemented with platelet-derived growth factor and basic fibroblast growth factor. OL progenitors were allowed to differentiate into pro-OLs and immature OLs under special cultural conditions. Cells at three different developmental stages were subjected to TNF-alpha treatment. Cell death, presumably by apoptosis as evidenced by TUNEL staining and caspase-3 activation, was observed following TNF-alpha treatment. Corresponding to TNF-alpha-induced apoptosis, cell survival rate decreased in a time- and dose-dependent manner. The sensitivity of different OL developmental stages to TNF-alpha decreased with the progression of cell maturation. However, this differential response was not related to differentially expressed TNF-alpha receptors. Consistent with reports that progenitor cells are preferentially injured in PVL, our results may further support the role of TNF-alpha as a potential mediator of PVL.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
TNFHumanperiventricular leukomalacia  ISOTnf (Rattus norvegicus) RGD 
TnfRatperiventricular leukomalacia  IDA  RGD 
TnfMouseperiventricular leukomalacia  ISOTnf (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Tnf  (tumor necrosis factor)

Genes (Mus musculus)
Tnf  (tumor necrosis factor)

Genes (Homo sapiens)
TNF  (tumor necrosis factor)


Additional Information