RGD Reference Report - Association of hepatic lipase with proteoglycans stimulates the production of proteoglycans in vivo and in vitro. - Rat Genome Database

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Association of hepatic lipase with proteoglycans stimulates the production of proteoglycans in vivo and in vitro.

Authors: Lee, SJ  Kadambi, S  David, C  Cooper, AD  Choi, SY 
Citation: Lee SJ, etal., J Lipid Res. 2004 Jul;45(7):1266-71. Epub 2004 Apr 21.
RGD ID: 1580511
Pubmed: (View Article at PubMed) PMID:15102889
DOI: Full-text: DOI:10.1194/jlr.M300372-JLR200

HL is synthesized in hepatocytes and functions while bound to heparan sulfate proteoglycans (HSPGs) in sinusoidal endothelial cells. The HL-mediated uptake of lipoprotein requires cell-surface HSPG. The present study tested whether HL plays a role in the production of HSPG. The production of HSPG in Chinese hamster ovary (CHO) cells was determined by measuring the incorporation of (35)SO(4) into PGs. HL-producing HL-CHO cells showed approximately 30% more cellular PG than did wild-type (WT) cells. In contrast, PG production in cells producing a membrane-anchored HL-glycophosphatidylinositol (GPI) that was not bound to HSPG was virtually identical to that in WT cells. When purified HL was added to the WT- or HL-GPI cells, PG production increased significantly to a level similar to that of the HL-secreting cells, suggesting that the binding of HL to HSPG triggered the increased HSPG production. Heparin reduced PG production in HL-producing cells, confirming that PG production is stimulated only when HL is present as a ligand for HSPG. Real-time PCR and Northern blots demonstrated that PG production was significantly reduced in animals lacking HL. Together, these data suggest that the binding of HL to PG on the cell surface exerts a positive feedback on cellular PG production.

Annotation

Gene Ontology Annotations    

Biological Process

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Lipc  (lipase C, hepatic type)


Additional Information