RGD Reference Report - Activation of the JAK-STAT signaling pathway in the rat basilar artery after subarachnoid hemorrhage. - Rat Genome Database

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Activation of the JAK-STAT signaling pathway in the rat basilar artery after subarachnoid hemorrhage.

Authors: Osuka, K  Watanabe, Y  Yamauchi, K  Nakazawa, A  Usuda, N  Tokuda, M  Yoshida, J 
Citation: Osuka K, etal., Brain Res. 2006 Feb 9;1072(1):1-7. Epub 2006 Jan 17.
RGD ID: 1580483
Pubmed: PMID:16413512   (View Abstract at PubMed)
DOI: DOI:10.1016/j.brainres.2005.12.003   (Journal Full-text)

The Janus kinase-signal transducer and activator of transcription (JAK-STAT) is one of the most important signaling pathways transducing signals from the cell surface in response to cytokines. Subarachnoid hemorrhage (SAH) produces cytokines in the CSF. We investigated whether this signaling pathway is activated in the rat basilar artery after SAH by cytokines. In a rat single-hemorrhage model of SAH, basilar arteries and CSF were obtained until 7 days after SAH. The concentration of interleukin-6 (IL-6) in CSF was measured by ELISA. Western blot analysis with JAK1, phosphospecific-JAK1, STAT3, phosphospecific STAT3 at Tyr705 and Ser727, cyclooxygenase-2 (COX-2), and actin antibodies was performed in basilar artery. The expressions of STAT3, phosphospecific STAT3 at Tyr705 and Ser727, and COX-2 in basilar artery were examined by immunohistochemical studies. The concentration of IL-6 immediately increased after SAH and Western blot analysis revealed that JAK1 was phosphorylated within 2 h, accompanied by phosphorylation of STAT3 at Tyr705, extending to Ser727 at days 1-2. Immunohistochemistry revealed phosphorylation of STAT3 to occur in endothelial and smooth muscle cells of the basilar artery. In addition, intracisternal injection of IL-6 by itself significantly increased phosphorylation of STAT3 at Tyr705 and Ser727. Expression of COX-2 was also upregulated in endothelial cells of the basilar artery. These results indicate that SAH produces the proinflammatory cytokine IL-6 in the CSF, which activates the JAK-STAT signaling pathway in the basilar artery and induces transcription of immediate early genes.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Il6Ratpositive regulation of receptor signaling pathway via JAK-STAT  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Il6Ratcytokine activity  IDA  RGD 
Il6Ratinterleukin-6 receptor binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il6  (interleukin 6)


Additional Information