RGD Reference Report - Regulation and role of the mitochondrial transcription factor in the diabetic rat heart. - Rat Genome Database

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Regulation and role of the mitochondrial transcription factor in the diabetic rat heart.

Authors: Nishio, Y  Kanazawa, A  Nagai, Y  Inagaki, H  Kashiwagi, A 
Citation: Nishio Y, etal., Ann N Y Acad Sci. 2004 Apr;1011:78-85.
RGD ID: 1578536
Pubmed: PMID:15126286   (View Abstract at PubMed)

To clarify the mechanism of abnormalities in mitochondrial expression and function in diabetic rat heart, we have studied the transcriptional activities of mitochondrial DNA using isolated intact mitochondria from the heart of either diabetic or control rats. The transcriptional activity of cardiac mitochondria isolated from diabetic rats decreased to 40% of the control level (P < 0.01). Consistently, in the heart of diabetic rats, the content of cytochrome b mRNA encoded by mitochondrial DNA was reduced to 50% of control (P < 0.01). This abnormal transcriptional activity of mitochondrial DNA could not be explained by mRNA or protein contents of mitochondrial transcription factor (mtTFA), but mtTFA binding to the promoter sequence of mitochondrial DNA, assessed by gel-shift assay, was attenuated in diabetic rats. In contrast, the mRNA expression of nuclear-encoded mitochondrial genes, such as ATP synthase-beta, was not affected by diabetes. Although O(2) consumption of the mitochondria from diabetic rats was decreased, H(2)O(2) production in these rats was increased compared with the control. Insulin treatment reversed all the abnormalities found in diabetic rats. These results clearly indicate that an impairment of binding activity of mtTFA to the promoter sequence has a key role in the abnormal mitochondrial gene expression, which might explain the mitochondrial dysfunction found in diabetic heart.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Diabetes Mellitus  ISOMt-cyb (Rattus norvegicus)1578536; 1578536mRNA:decreased expression:heartRGD 
Experimental Diabetes Mellitus  IEP 1578536mRNA:decreased expression:heartRGD 

Objects Annotated

Genes (Rattus norvegicus)
Mt-cyb  (mitochondrially encoded cytochrome b)

Genes (Mus musculus)
mt-Cytb  (cytochrome b, mitochondrial)

Genes (Homo sapiens)
MT-CYB  (mitochondrially encoded cytochrome b)

Objects referenced in this article
Gene Tfam transcription factor A, mitochondrial Rattus norvegicus

Additional Information