RGD Reference Report - The role of polyamines in growth factor induced DNA synthesis in cultured rat hepatocytes. - Rat Genome Database

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The role of polyamines in growth factor induced DNA synthesis in cultured rat hepatocytes.

Authors: Higaki, I  Matsui-Yuasa, I  Hirohashi, K  Kinoshita, H  Otani, S 
Citation: Higaki I, etal., Hepatogastroenterology. 1999 May-Jun;46(27):1874-9.
RGD ID: 1578320
Pubmed: PMID:10430362   (View Abstract at PubMed)

BACKGROUND/AIMS: Hepatocyte growth factor and transforming growth factor-alpha are growth factors with important roles in hepatocyte proliferation. The polyamines, putrescine, spermidine, and spermine are widely distributed in many different cells and play an essential role in cell growth and differentiation. The present study examined the role of polyamine in this growth promoting factor-induced hepatocyte proliferation, in primary cultured rat hepatocytes. METHODOLOGY: Hepatocytes were isolated from rats by the collagenase perfusion method. Ornithine decarboxylase and S-adenosylmethionine decarboxylase activities were measured as the release of 14CO2 from L-[-14C]ornithine and S-adenosyl-L-[carboxyl14C]methionine, respectively. The concentration of polyamine was analyzed by high performance liquid chromatography. RESULTS: When transforming growth factor-alpha and hepatocyte growth factor were added to the hepatocyte culture simultaneously, ornithine decarboxylase activity, S-adenosylmethionine decarboxylase activity, polyamine concentration and DNA synthesis increased additively. The increase in DNA synthesis caused by transforming growth factor-alpha, hepatocyte growth factor, or both was completely inhibited by alpha-difluoromethylornithine and methylglyoxal bis(guanylhydrazone). The inhibition was reversed by exogenous spermidine or spermine, but not by putrescine. CONCLUSIONS: Increased spermidine or spermine levels are essential for hepatocyte proliferation in cultured rat hepatocytes.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Amd1Ratpolyamine metabolic process  IDA  RGD 
Odc1Ratpolyamine metabolic process  IDA  RGD 
Odc1Ratputrescine biosynthetic process  IDA  RGD 
Amd1Ratspermidine biosynthetic process  IDA  RGD 
Amd1Ratspermine biosynthetic process  IDA  RGD 

Molecular Function

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Amd1Ratadenosylmethionine decarboxylase activity  IDA  RGD 
Odc1Ratornithine decarboxylase activity  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

Objects Annotated

Genes (Rattus norvegicus)
Amd1  (adenosylmethionine decarboxylase 1)
Odc1  (ornithine decarboxylase 1)

Genes (Mus musculus)
Amd1  (S-adenosylmethionine decarboxylase 1)
Odc1  (ornithine decarboxylase, structural 1)

Genes (Homo sapiens)
AMD1  (adenosylmethionine decarboxylase 1)
ODC1  (ornithine decarboxylase 1)

Additional Information