RGD Reference Report - Spliceosome protein EFTUD2 is upregulated in the trophoblast of spontaneous miscarriage and hydatidiform mole. - Rat Genome Database

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Spliceosome protein EFTUD2 is upregulated in the trophoblast of spontaneous miscarriage and hydatidiform mole.

Authors: Löb, Sanja  Vattai, Aurelia  Kuhn, Christina  Schmoeckel, Elisa  Mahner, Sven  Wöckel, Achim  Kolben, Thomas  Szekeres-Bartho, Julia  Jeschke, Udo  Vilsmaier, Theresa 
Citation: Löb S, etal., J Reprod Immunol. 2020 Aug;140:103149. doi: 10.1016/j.jri.2020.103149. Epub 2020 May 18.
RGD ID: 155791666
Pubmed: PMID:32447180   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jri.2020.103149   (Journal Full-text)


BACKGROUND: Elongation factor Tu GTP binding domain containing 2 (EFTUD2) is an alternative splicing factor that modulates cell differentiation and activation processes. EFTUD2 is known to modulate immune responses and mutation of the EFTUD2-gene lead to fetal malformation. Little is known about its expression and role in normal and disturbed first trimester pregnancy.
PATIENTS AND METHODS: We investigated the expression of EFTUD2 in placental tissue obtained from patients with normal (n = 14), spontaneous miscarriage (n = 15) and molar (n = 14) pregnancy by immunohistochemistry. The expression of EFTUD2 was correlated on the protein level with known immune modulatory proteins like pregnancy zone protein (PZP) and in addition with human chorionic gonadotropin (hCG). Furthermore, we analysed the EFTUD2 and PZP expression in vitro after stimulation of the chorioncarcinoma cell line JEG-3 with hCG.
RESULTS: EFTUD2 is significantly upregulated in the syncytiotrophoblast of spontaneous miscarriage (p = 0.003) and molar pregnancy (p = 0.003) compared to week of gestation-adjusted normal first trimester placentas. PZP is negatively correlated (p = 0.021) to EFTUD2 in the syncytiotrophoblast and is therefore significantly downregulated in miscarriage (p = 0.028) and mole pregnancy (p = 0.006). In addition, hCG is positively correlated to EFTUD2 in mole pregnancy. The addition of hCG to chorioncarcinoma cell lines JEG-3 in vitro stimulated EFTUD2 expression in these cells (p = 0.027).
CONCLUSION: Regulation of alternative splicing seems crucial for a successful ongoing pregnancy. The up-regulated elongation factor EFTUD2 may have a critical role in miscarriage.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
EFTUD2HumanHydatidiform Mole  IEP protein:increased expression:trophoblast and decidua:RGD 
Eftud2MouseHydatidiform Mole  ISOEFTUD2 (Homo sapiens)protein:increased expression:trophoblast and decidua:RGD 
Eftud2RatHydatidiform Mole  ISOEFTUD2 (Homo sapiens)protein:increased expression:trophoblast and decidua:RGD 
EFTUD2HumanSpontaneous Abortions  IEP protein:increased expression:trophoblast and decidua:RGD 
Eftud2MouseSpontaneous Abortions  ISOEFTUD2 (Homo sapiens)protein:increased expression:trophoblast and decidua:RGD 
Eftud2RatSpontaneous Abortions  ISOEFTUD2 (Homo sapiens)protein:increased expression:trophoblast and decidua:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Eftud2  (elongation factor Tu GTP binding domain containing 2)

Genes (Mus musculus)
Eftud2  (elongation factor Tu GTP binding domain containing 2)

Genes (Homo sapiens)
EFTUD2  (elongation factor Tu GTP binding domain containing 2)


Additional Information