RGD Reference Report - A subtractive gene expression screen suggests a role of transcription factor AP-2 alpha in control of proliferation and differentiation. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

A subtractive gene expression screen suggests a role of transcription factor AP-2 alpha in control of proliferation and differentiation.

Authors: Pfisterer, P  Ehlermann, J  Hegen, M  Schorle, H 
Citation: Pfisterer P, etal., J Biol Chem 2002 Feb 22;277(8):6637-44. Epub 2001 Dec 10.
RGD ID: 1556631
Pubmed: PMID:11741941   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M108578200   (Journal Full-text)

The transcription factor AP-2 alpha has been implicated as a cell type-specific regulator of gene expression during vertebrate embryogenesis based on its expression pattern in neural crest cells, ectoderm, and the nervous system in mouse and frog embryos. AP-2 alpha is prominently expressed in cranial neural crest cells, a population of cells that migrate from the lateral margins of the brain plate during closure of the neural tube at day 8-9 of embryonic development. Homozygous AP-2 alpha mutant mice die perinatally with cranio-abdominoschisis, full facial clefting, and defects in cranial ganglia and sensory organs, indicating the importance of this gene for proper development. By using a subtractive cloning approach, we identified a set of genes repressed by AP-2 alpha that are described to retard cellular proliferation and induce differentiation and apoptosis. We show that these target genes are prematurely expressed in AP-2 alpha mutant mice. One of the genes isolated, the Kruppel-box transcription factor KLF-4 implicated in induction of terminal differentiation and growth regulation, is found expressed in mutant embryonic fibroblasts. We show that fibroblasts lacking AP-2 alpha display retarded growth but no enhanced apoptosis. Based on these data we suggest that AP-2 alpha might be required for cell proliferation by suppression of genes inducing terminal differentiation, apoptosis, and growth retardation.

Objects referenced in this article
Gene Anxa1 annexin A1 Mus musculus
Gene Bhlhe40 basic helix-loop-helix family, member e40 Mus musculus
Gene Blcap bladder cancer associated protein Mus musculus
Gene Bok BCL2-related ovarian killer Mus musculus
Gene Brpf1 bromodomain and PHD finger containing, 1 Mus musculus
Gene Gpx3 glutathione peroxidase 3 Mus musculus
Gene Grpel1 GrpE-like 1, mitochondrial Mus musculus
Gene Klf4 Kruppel-like transcription factor 4 (gut) Mus musculus
Gene Ly6a lymphocyte antigen 6 family member A Mus musculus
Gene Mcm5 minichromosome maintenance complex component 5 Mus musculus
Gene mt-Co1 cytochrome c oxidase I, mitochondrial Mus musculus
Gene Olfml3 olfactomedin-like 3 Mus musculus
Gene Pabpn1 poly(A) binding protein, nuclear 1 Mus musculus
Gene Prdx1 peroxiredoxin 1 Mus musculus
Gene Prl8a2 prolactin family 8, subfamily a, member 2 Mus musculus
Gene Psap prosaposin Mus musculus
Gene Psmc2 proteasome (prosome, macropain) 26S subunit, ATPase 2 Mus musculus
Gene Rchy1 ring finger and CHY zinc finger domain containing 1 Mus musculus
Gene Rpl18 ribosomal protein L18 Mus musculus
Gene Rpl5 ribosomal protein L5 Mus musculus
Gene Rpl6 ribosomal protein L6 Mus musculus
Gene Rpl7 ribosomal protein L7 Mus musculus
Gene Rps5 ribosomal protein S5 Mus musculus
Gene Selenop selenoprotein P Mus musculus
Gene Stx7 syntaxin 7 Mus musculus
Gene Tmbim6 transmembrane BAX inhibitor motif containing 6 Mus musculus

Additional Information