RGD Reference Report - Trans-resveratrol imparts disparate effects on transcription of DNA damage sensing/repair pathway genes in euglycemic and hyperglycemic rat testis. - Rat Genome Database

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Trans-resveratrol imparts disparate effects on transcription of DNA damage sensing/repair pathway genes in euglycemic and hyperglycemic rat testis.

Authors: Kilarkaje, Narayana  Al-Qaryyan, Mariam  Al-Bader, Maie D 
Citation: Kilarkaje N, etal., Toxicol Appl Pharmacol. 2021 May 1;418:115510. doi: 10.1016/j.taap.2021.115510. Epub 2021 Mar 26.
RGD ID: 155631260
Pubmed: PMID:33775663   (View Abstract at PubMed)
DOI: DOI:10.1016/j.taap.2021.115510   (Journal Full-text)

Prevention or repair of DNA damage is critical to inhibit carcinogenesis in living organisms. Using quantitative RT2 Profiler™ PCR array, we investigated if trans-resveratrol could modulate the transcription of DNA damage sensing/repair pathway genes in euglycemic and non-obese type 2 diabetic Goto-Kakizaki rat testis. Trans-resveratrol imparted disparate effects on gene expressions. In euglycemic rats, it downregulated 79% and upregulated 2% of genes. However, in diabetic rats, it upregulated only 2% and downregulated 4% of genes. As such, diabetes upregulated 16% and downregulated 4% of genes. Trans-resveratrol normalized the expression of 9 (60%) out of 15 upregulated genes in diabetic rats. In euglycemic rats, trans-resveratrol inhibited ATM/ATR, DNA damage repair, pro-cell cycle progression, and apoptosis signaling genes. However, it increased Cdkn1a and Sumo1, indicating cell cycle arrest, apoptosis, and cytostasis in conjunction with increased DNA double-strand breaks and apoptosis. Diabetes increased DNA damage and apoptosis but did not affect ATM/ATR and double-strand break repair genes, although it increased few single-strand repair genes. Diabetes increased Abl1 and Sirt1, which may be related to apoptosis, but their increase may well suggest the enhanced cell cycle progression and putative carcinogenicity. The transcription of Rad17 and Smc1a increased in diabetic rats indicating G2 phase arrest and increases in a few DNA single-strand breaks repair genes suggesting DNA damage repair. Trans-resveratrol inhibits the cell cycle and causes cell death in euglycemic rat testis but normalizes diabetes-induced genes related to DNA damage and cell cycle control, suggesting its usefulness in maintaining DNA integrity in diabetes.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
type 2 diabetes mellitus  ISOSmc1a (Rattus norvegicus)155631260; 155631260mRNA:increased expression:testis:RGD 
type 2 diabetes mellitus  IEP 155631260mRNA:increased expression:testis:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Smc1a  (structural maintenance of chromosomes 1A)

Genes (Mus musculus)
Smc1a  (structural maintenance of chromosomes 1A)

Genes (Homo sapiens)
SMC1A  (structural maintenance of chromosomes 1A)


Additional Information