RGD Reference Report - Identification of a mammalian vesicular polyamine transporter. - Rat Genome Database

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Identification of a mammalian vesicular polyamine transporter.

Authors: Hiasa, Miki  Miyaji, Takaaki  Haruna, Yuka  Takeuchi, Tomoya  Harada, Yuika  Moriyama, Sawako  Yamamoto, Akitsugu  Omote, Hiroshi  Moriyama, Yoshinori 
Citation: Hiasa M, etal., Sci Rep. 2014 Oct 30;4:6836. doi: 10.1038/srep06836.
RGD ID: 155230738
Pubmed: PMID:25355561   (View Abstract at PubMed)
PMCID: PMC4213795   (View Article at PubMed Central)
DOI: DOI:10.1038/srep06836   (Journal Full-text)

Spermine and spermidine act as neuromodulators upon binding to the extracellular site(s) of various ionotropic receptors, such as N-methyl-d-aspartate receptors. To gain access to the receptors, polyamines synthesized in neurons and astrocytes are stored in secretory vesicles and released upon depolarization. Although vesicular storage is mediated in an ATP-dependent, reserpine-sensitive fashion, the transporter responsible for this process remains unknown. SLC18B1 is the fourth member of the SLC18 transporter family, which includes vesicular monoamine transporters and vesicular acetylcholine transporter. Proteoliposomes containing purified human SLC18B1 protein actively transport spermine and spermidine by exchange of H(+). SLC18B1 protein is predominantly expressed in the hippocampus and is associated with vesicles in astrocytes. SLC18B1 gene knockdown decreased both SLC18B1 protein and spermine/spermidine contents in astrocytes. These results indicated that SLC18B1 encodes a vesicular polyamine transporter (VPAT).



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc18a2Ratserotonin uptake involved_inIDA PMID:25355561UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc18b1Ratsynaptic vesicle membrane located_inIDA PMID:25355561UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc18a2Ratmonoamine:proton antiporter activity enablesIDA PMID:25355561UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Slc18a2  (solute carrier family 18 member A2)
Slc18b1  (solute carrier family 18 member B1)


Additional Information