RGD Reference Report - Subcellular localization of GABAB receptor subunits in rat globus pallidus. - Rat Genome Database

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Subcellular localization of GABAB receptor subunits in rat globus pallidus.

Authors: Chen, Lei  Boyes, Justin  Yung, Wing-Ho  Bolam, J Paul 
Citation: Chen L, etal., J Comp Neurol. 2004 Jun 28;474(3):340-52. doi: 10.1002/cne.20143.
RGD ID: 155230705
Pubmed: PMID:15174078   (View Abstract at PubMed)
DOI: DOI:10.1002/cne.20143   (Journal Full-text)

The inhibitory amino acid gamma-aminobutyric acid (GABA) is the major neurotransmitter in the globus pallidus. Although electrophysiological studies have indicated that functional GABA(B) receptors exist in rat globus pallidus, the subcellular localization of GABA(B) receptor subunits and their spatial relationship to glutamatergic and GABAergic synapses are unknown. Here, we use pre-embedding immunogold labeling to study the subcellular localization of GABA(B) receptor subunits, GABA(B1) and GABA(B2), in globus pallidus neurons and identified populations of afferent terminals. Immunolabeling for GABA(B1) and GABA(B2) was observed throughout the globus pallidus, with GABA(B1) more strongly expressed in perikarya and GABA(B2) mainly expressed in the neuropil. Electron microscopic analysis revealed that the majority of GABA(B1) labeling was localized within the cytoplasm, whereas most of GABA(B2) labeling was associated with the plasma membrane. At the subcellular level, both the GABA(B1) and GABA(B2) immunogold labeling was localized at pre- and postsynaptic sites. At asymmetric, putative excitatory, synapses, GABA(B1) and GABA(B2) immunogold labeling was found at perisynaptic sites of both pre- and postsynaptic specializations. Double immunolabeling, using the vesicular glutamate transporter 2 (VGLUT2), revealed the glutamatergic nature of most immunogold-labeled asymmetric synapses. At symmetric, putative GABAergic, synapses, including those formed by anterogradely labeled striatopallidal terminals, GABA(B1) and GABA(B2) immunogold labeling was found in the main body of both pre- and postsynaptic specializations. These results demonstrate the existence of presynaptic GABA(B) auto- and heteroreceptors and postsynaptic GABA(B) receptors, which may be involved in modulating synaptic transmission in the globus pallidus.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gabrb1RatGABA-ergic synapse is_active_inIDA PMID:15174078SynGO 
Gabrb2RatGABA-ergic synapse is_active_inIDA PMID:15174078SynGO 
Gabrb2Ratglutamatergic synapse is_active_inIDA PMID:15174078SynGO 
Gabrb1Ratpostsynaptic specialization membrane is_active_inIDA PMID:15174078SynGO 
Gabrb2Ratpostsynaptic specialization membrane is_active_inIDA PMID:15174078SynGO 
Gabrb1Ratpresynaptic active zone membrane is_active_inIDA PMID:15174078SynGO 
Gabrb2Ratpresynaptic active zone membrane is_active_inIDA PMID:15174078SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Gabrb1  (gamma-aminobutyric acid type A receptor subunit beta1)
Gabrb2  (gamma-aminobutyric acid type A receptor subunit beta 2)


Additional Information