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A novel RNA-binding nuclear protein that interacts with the fragile X mental retardation (FMR1) protein.

Authors: Bardoni, B  Schenck, A  Mandel, JL 
Citation: Bardoni B, etal., Hum Mol Genet 1999 Dec;8(13):2557-66.
Pubmed: (View Article at PubMed) PMID:10556305

Silenced expression of the FMR1 gene is responsible for the fragile X syndrome. The FMR1 gene codes for an RNA binding protein (FMRP), which can shuttle between the nucleus and the cytoplasm and is found associated to polysomes in the cytoplasm. By two-hybrid assay in yeast, we identified a novel protein interacting with FMRP: nuclear FMRP interacting protein (NUFIP). NUFIP mRNA expression is strikingly similar to that of the FMR1 gene in neurones of cortex, hippocampus and cerebellum. At the subcellular level, NUFIP colocalizes with nuclear isoforms of FMRP in a dot-like pattern. NUFIP presents a C2H2 zinc finger motif and a nuclear localization signal, but has no homology to known proteins and shows RNA binding activity in vitro. NUFIP does not interact with the FMRP homologues encoded by the FXR1 and FXR2 genes. Thus, these results indicate a specific nuclear role for FMRP.


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RGD Object Information
RGD ID: 1549875
Created: 2005-09-25
Species: All species
Last Modified: 2005-09-25
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.