RGD Reference Report - Quantitative trait loci mapping for intracellular calcium in spontaneously hypertensive rats. - Rat Genome Database

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Quantitative trait loci mapping for intracellular calcium in spontaneously hypertensive rats.

Authors: Ohno, Y  Suzuki, H  Tanase, H  Otsuka, K  Sasaki, T  Suzawa, T  Morii, T  Ando, Y  Maruyama, T  Saruta, T 
Citation: Ohno Y, etal., Am J Hypertens 2005 May;18(5 Pt 1):666-71.
RGD ID: 1547855
Pubmed: (View Article at PubMed) PMID:15882549
DOI: Full-text: DOI:10.1016/j.amjhyper.2004.12.001

BACKGROUND: Increased intracellular calcium ([Ca2+]i) in platelets is also proposed as an intermediate phenotype for hypertension in spontaneously hypertensive rats (SHR). Increased [Ca2+]i in platelets is hypothesized to contribute to atherothrombotic events. Platelet hyperactivity is frequently associated with cardiovascular disease. METHODS: In a genome scan, we performed the quantitative trait loci (QTL) mapping for [Ca2+]i in back-crossed rats derived from SHR and normotensive Fischer 344 rats, which demonstrated a single major QTL for hypertension on chromosome 1. Thrombin-stimulated [Ca2+]i in Ca2+-free and in Ca2+-containing buffers was measured in platelets using the Fura-2 method. RESULTS: Among the parental strains, systolic blood pressure and thrombin-stimulated [Ca2+]i were significantly greater in SHR than in Fischer 344 and F1 rats. The sarco(endo)plasmic reticulum Ca2+-dependent ATPase II gene locus (Serca2) between D12Mgh5 and D12Mgh6 showed the significant linkage for thrombin-stimulated [Ca2+]i in Ca2+-free and Ca2+-containing buffers. The peak logarithm of the odds scores were 3.6 and 3.3, respectively. These QTL explained 19.8% and 17.4% of the total variances, respectively. D3Mit13 and DXMgh1 showed suggestive linkage for thrombin-stimulated [Ca2+]i in Ca2+-free and in Ca2+-containing buffers, respectively. The peak logarithm of the odds scores were 2.6 and 2.1, respectively. CONCLUSIONS: A significant QTL for [Ca2+]i was mapped near Serca2 on chromosome 12, and suggestive QTL were identified near D3Mit13 and DXMgh1 in a genome scan. Genetic abnormalites in platelet [Ca2+]i may contribute to cardiovascular disease via platelet hyperactivity, independent of blood pressure elevation.

Annotation

Disease Annotations    
hypertension  (IDA)

Objects Annotated

QTLs
Calcic1  (Intracellular calcium level QTL 1)

Objects referenced in this article
0 F344/Snk null All species

Additional Information