RGD Reference Report - Analysis of angiogenic markers in oral squamous cell carcinoma-gene and protein expression. - Rat Genome Database

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Analysis of angiogenic markers in oral squamous cell carcinoma-gene and protein expression.

Authors: Jung, Susanne  Sielker, Sonja  Purcz, Nikolai  Sproll, Christoph  Acil, Yahya  Kleinheinz, Johannes 
Citation: Jung S, etal., Head Face Med. 2015 Jun 5;11:19. doi: 10.1186/s13005-015-0076-7.
RGD ID: 153323290
Pubmed: PMID:26044849   (View Abstract at PubMed)
PMCID: PMC4461981   (View Article at PubMed Central)
DOI: DOI:10.1186/s13005-015-0076-7   (Journal Full-text)


PURPOSE: Therapeutic strategies attacking oral squamous cell carcinoma have not essentially succeeded to improve long-term prognosis and overall survival over the last decades. Therefore, in this study, we aimed to illuminate the molecular regulation of angiogenesis in this tumour entity in order to demask novel markers of prognosis or therapeutic approach.
MATERIALS AND METHODS: A panel of significant transcriptional alterations in angiogenic genes of 83 cancer samples was established by comparison to 30 samples of healthy oral mucosa with microarray technique. Immunohistochemistry (IHC) was performed to trace the signalling cascade from gene to protein level.
RESULTS: A distinctive expression profile of VEGFA, EFNB2, PECAM1/CD31, ANGPT1 and ANGPT2 was revealed: VEGFA, EFNB2, and ANGPT2 were found overexpressed in 84 % to 95 % of tumour samples. In contrast, the expression of CD31 and ANGPT1 was downregulated in 80 % to 95 % of tumour samples. IHC confirmed results of the microarray analysis. Tumours with lymphatic spread showed higher gene expression rates of VEGFA, EFNB2 and ANGPT2 in moderately differentiated tumours and of VEGFA and EFNB2 in small tumours, respectively. The ANGPT1/ ANGPT2 transcription ratio was found decreased in larger tumours and especially in tumours without lymphatic spread.
CONCLUSIONS: A characteristic expression profile of angiogenic markers was established. The specific overexpression of EFNB2 in small tumours with lymphatic spread and the typical decrease of the ANGPT1/ ANGPT2 ratio in larger tumours give weight to EFNB2 and angiopoietins as prognostic factors and potential therapeutic targets.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
oral squamous cell carcinoma  HEP 153323290; 153323290protein:decreased expression:oral mucosa (human)RGD 
oral squamous cell carcinoma  HEP 153323290; 153323290; 153323290protein:increased expression:oral mucosa (human)RGD 

Objects Annotated

Genes (Homo sapiens)
ANGPT1  (angiopoietin 1)
ANGPT2  (angiopoietin 2)
EFNB2  (ephrin B2)
PECAM1  (platelet and endothelial cell adhesion molecule 1)
VEGFA  (vascular endothelial growth factor A)


Additional Information