RGD Reference Report - MicroRNA-31 predicts the presence of lymph node metastases and survival in patients with lung adenocarcinoma. - Rat Genome Database

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MicroRNA-31 predicts the presence of lymph node metastases and survival in patients with lung adenocarcinoma.

Authors: Meng, Wei  Ye, Zhenqing  Cui, Ri  Perry, James  Dedousi-Huebner, Vaia  Huebner, Alexander  Wang, Yao  Li, Bin  Volinia, Stefano  Nakanishi, Hiroshi  Kim, Taewan  Suh, Sung-Suk  Ayers, Leona W  Ross, Patrick  Croce, Carlo M  Chakravarti, Arnab  Jin, Victor X  Lautenschlaeger, Tim 
Citation: Meng W, etal., Clin Cancer Res. 2013 Oct 1;19(19):5423-33. doi: 10.1158/1078-0432.CCR-13-0320. Epub 2013 Aug 14.
RGD ID: 152998944
Pubmed: PMID:23946296   (View Abstract at PubMed)
PMCID: PMC3823052   (View Article at PubMed Central)
DOI: DOI:10.1158/1078-0432.CCR-13-0320   (Journal Full-text)


PURPOSE: We conducted genome-wide miRNA-sequencing (miRNA-seq) in primary cancer tissue from patients of lung adenocarcinoma to identify markers for the presence of lymph node metastasis.
EXPERIMENTAL DESIGN: Markers for lymph node metastasis identified by sequencing were validated in a separate cohort using quantitative PCR. After additional validation in the The Cancer Genome Atlas (TCGA) dataset, functional characterization studies were conducted in vitro.
RESULTS: MiR-31 was upregulated in lung adenocarcinoma tissues from patients with lymph node metastases compared with those without lymph node metastases. We confirmed miR-31 to be upregulated in lymph node-positive patients in a separate patient cohort (P = 0.009, t test), and to be expressed at higher levels in adenocarcinoma tissue than in matched normal adjacent lung tissues (P < 0.0001, paired t test). MiR-31 was then validated as a marker for lymph node metastasis in an external validation cohort of 233 lung adenocarcinoma cases of the TCGA (P = 0.031, t test). In vitro functional assays showed that miR-31 increases cell migration, invasion, and proliferation in an ERK1/2 signaling-dependent manner. Notably, miR-31 was a significant predictor of survival in a multivariate cox regression model even when controlling for cancer staging. Exploratory in silico analysis showed that low expression of miR-31 is associated with excellent survival for T2N0 patients.
CONCLUSIONS: We applied miRNA-seq to study microRNomes in lung adenocarcinoma tissue samples for the first time and potentially identified a miRNA predicting the presence of lymph node metastasis and survival outcomes in patients of lung adenocarcinoma.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
lung adenocarcinoma disease_progressionIEP 152998944RNA:increased expression:lung (human)RGD 
lung adenocarcinoma disease_progressionISOMIR31 (Homo sapiens)152998944; 152998944RNA:increased expression:lung (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir31  (microRNA 31)

Genes (Mus musculus)
Mir31  (microRNA 31)

Genes (Homo sapiens)
MIR31  (microRNA 31)


Additional Information