RGD Reference Report - The sodium-bicarbonate cotransporter NBCe1 supports glutamine efflux via SNAT3 (SLC38A3) co-expressed in Xenopus oocytes. - Rat Genome Database

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The sodium-bicarbonate cotransporter NBCe1 supports glutamine efflux via SNAT3 (SLC38A3) co-expressed in Xenopus oocytes.

Authors: Wendel, Christina  Becker, Holger M  Deitmer, Joachim W 
Citation: Wendel C, etal., Pflugers Arch. 2008 Feb;455(5):885-93. doi: 10.1007/s00424-007-0351-y. Epub 2007 Oct 2.
RGD ID: 152995561
Pubmed: PMID:17909850   (View Abstract at PubMed)
DOI: DOI:10.1007/s00424-007-0351-y   (Journal Full-text)

The glutamine transporter SNAT3 contributes to the glutamine fluxes in liver, kidney, and brain. We heterologously co-expressed SNAT3 with the electrogenic sodium-bicarbonate cotransporter NBCe1 in Xenopus laevis oocytes and measured cytosolic pH and membrane current in voltage clamp. Because of the increased buffer capacity contributed by the NBCe1 (Becker and Deitmer in J Biol Chem 279:28057-28062, 2004), we hypothesized that this may enhance the proton-coupled glutamine transport via SNAT3 in the presence of CO2/HCO3-. Addition and removal of glutamine activated not only SNAT3 but also NBCe1, as indicated by the increased membrane current. The NBCe1 current during glutamine removal was more than 50% larger than during glutamine addition, suggesting that NBCe1 enhances glutamine efflux rather than glutamine uptake. This was confirmed by radio-labeled glutamine flux measurements; influx of glutamine was significantly decreased, whereas efflux of glutamine was increased when SNAT3 was co-expressed with NBCe1. A model is presented that attempts to explain the role of intracellular pH, bicarbonate transport, and buffering capacity mediated by NBCe1 for uptake and efflux of glutamine via SNAT3.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc38a3Ratglutamine secretion involved_inIDA PMID:17909850UniProt 
Slc38a3RatL-glutamine import across plasma membrane involved_inIDA PMID:17909850UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Slc38a3  (solute carrier family 38, member 3)


Additional Information