RGD Reference Report - Identification and purification of the tricarboxylate carrier from rat liver mitochondria. - Rat Genome Database

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Identification and purification of the tricarboxylate carrier from rat liver mitochondria.

Authors: Bisaccia, F  De Palma, A  Palmieri, F 
Citation: Bisaccia F, etal., Biochim Biophys Acta. 1989 Nov 23;977(2):171-6. doi: 10.1016/s0005-2728(89)80068-4.
RGD ID: 152995536
Pubmed: PMID:2804096   (View Abstract at PubMed)
DOI: DOI:10.1016/s0005-2728(89)80068-4   (Journal Full-text)

The tricarboxylate carrier from rat liver mitochondria was solubilized with Triton X-100 and purified by chromatography on hydroxyapatite and celite. SDS-gel electrophoresis of the purified fraction showed a single polypeptide band with an apparent Mr of 30,000. When reconstituted into liposomes, the tricarboxylate transport protein catalyzed a 1,2,3-benzenetricarboxylate-sensitive citrate/citrate exchange. We obtained a 1070-fold purification with respect to the mitochondrial extract, the recovery was 22% and the protein yield 0.02%. The properties of the reconstituted carrier, i.e., requirement for a counteranion, substrate specificity and inhibitor sensitivity, were similar to those of the tricarboxylate transport system as characterized in intact mitochondria.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc25a1Ratmitochondrial citrate transmembrane transport involved_inIDA PMID:2804096UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc25a1Ratcitrate secondary active transmembrane transporter activity enablesIDA PMID:2804096UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Slc25a1  (solute carrier family 25 member 1)


Additional Information