RGD Reference Report - MiR-154-5p Suppresses Cell Invasion and Migration Through Inhibiting KIF14 in Nasopharyngeal Carcinoma. - Rat Genome Database

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MiR-154-5p Suppresses Cell Invasion and Migration Through Inhibiting KIF14 in Nasopharyngeal Carcinoma.

Authors: Chen, Jie  Ma, Chengxian  Zhang, Yufeng  Pei, Shuai  Du, Mingyu  Zhang, Yujie  Qian, Luxi  Wang, Jianlin  Yin, Li  He, Xia 
Citation: Chen J, etal., Onco Targets Ther. 2020 Mar 13;13:2235-2246. doi: 10.2147/OTT.S242939. eCollection 2020.
RGD ID: 152995464
Pubmed: PMID:32214824   (View Abstract at PubMed)
PMCID: PMC7078655   (View Article at PubMed Central)
DOI: DOI:10.2147/OTT.S242939   (Journal Full-text)


BACKGROUND: Mounting evidence has reported that microRNA-154-5p (miR-154-5p) is involved in the development of multiple cancers, but its function in nasopharyngeal carcinoma (NPC) remains not well investigated.
METHODS: Real-time quantitative PCR (qRT-PCR) was used to detect miR-154-5p expression in NPC tissues and cells. CCK8, colony formation, wound healing and transwell assays were performed to assess cell proliferation, migration and invasion. Dual-luciferase reporter assays and Western blots were performed to confirm the target gene of miR-154-5p. Rescue experiments were conducted to explore the influence of target gene KIF14 on the functions of miR-154-5p. Xenograft tumor model was conducted to detect the effect of miR-154-5p in vivo.
RESULTS: qRT-PCR results revealed that the expression of miR-154-5p was down-regulated in NPC tissues and cell lines compared to normal nasopharyngeal tissues and cell line. Overexpression of miR-154-5p inhibited cell migration and invasion. However, miR-154-5p had no influence on the proliferation of NPC cells. MiR-154-5p overexpression suppressed xenograft tumor metastasis in vivo. Dual-luciferase reporter analysis identified KIF14 as a target gene of miR-154-5p. Rescue experiments showed that knockdown of KIF14 reversed the effect of inhibiting miR-154-5p expression on NPC cell migration and invasion.
CONCLUSION: Taken together, miR-154-5p suppresses tumor migration and invasion by targeting KIF14 in NPC. The newly identified miR-154-5p/KIF14 interaction offers further insights into the progression of NPC, which may represent a novel target for NPC diagnosis and treatment.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
nasopharynx carcinoma disease_progressionIEP 152995464RNA:decreased expression:epithelium of nasopharynx (human)RGD 
nasopharynx carcinoma disease_progressionISOMIR154 (Homo sapiens)152995464; 152995464RNA:decreased expression:epithelium of nasopharynx (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir154  (microRNA 154)

Genes (Mus musculus)
Mir154  (microRNA 154)

Genes (Homo sapiens)
MIR154  (microRNA 154)


Additional Information