RGD Reference Report - MicroRNA-127 is a tumor suppressor in human esophageal squamous cell carcinoma through the regulation of oncogene FMNL3. - Rat Genome Database

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MicroRNA-127 is a tumor suppressor in human esophageal squamous cell carcinoma through the regulation of oncogene FMNL3.

Authors: Gao, Xuhui  Wang, Xuelian  Cai, Kaican  Wang, Wujun  Ju, Qun  Yang, Xiyao  Wang, Haofei  Wu, Hua 
Citation: Gao X, etal., Eur J Pharmacol. 2016 Nov 15;791:603-610. doi: 10.1016/j.ejphar.2016.09.025. Epub 2016 Sep 17.
RGD ID: 152995354
Pubmed: PMID:27645894   (View Abstract at PubMed)
DOI: DOI:10.1016/j.ejphar.2016.09.025   (Journal Full-text)

In this study, we investigated the expression patterns and functional roles of microRNA 127 (miR-127) and its target gene Formin-Like 3 (FMNL3) in human esophageal squamous cell carcinoma (ESCC). Quantitative RT-PCR (qRT-PCR) was used to compare miR-127 expression between ESCC cell lines and normal esophageal epithelium cell line, as well as paired ESCC tumors and adjacent normal esophageal tissues in 33 patients. We found miR-127 was aberrantly downregulated in both ESCC cell lines and human ESCC tumors. In ESCC cell lines TE-1 and ECA109 cells, lentiviral-induced miR-127 upregulation markedly inhibited cancer proliferation and migration in vitro, and tumorigenicity in vivo. Through dual-luciferase assay and qRT-PCR, FMNL3 was confirmed to be the downstream target gene of miR-127 in ESCC. Finally, FMNL3 was downregulated by siRNA in TE-1 and ECA109 cells. And we discovered that SiRNA-induced FMNL3 downregulation had tumor suppressive effect in ESCC, inhibiting cancer proliferation, migration in vitro, and tumorigenicity in vivo. These results suggest that miR-127 is downregulated and acting as tumor suppressor in ESCC. Inversely, FMNL3, the target gene of miR-127, is upregulated and acting as an oncogene in ESCC.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MIR127Humanesophagus squamous cell carcinoma  IEP RNA:decreased expression:esophagus (human)RGD 
Mir127Mouseesophagus squamous cell carcinoma  ISOMIR127 (Homo sapiens)RNA:decreased expression:esophagus (human)RGD 
Mir127Ratesophagus squamous cell carcinoma  ISOMIR127 (Homo sapiens)RNA:decreased expression:esophagus (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir127  (microRNA 127)

Genes (Mus musculus)
Mir127  (microRNA 127)

Genes (Homo sapiens)
MIR127  (microRNA 127)


Additional Information