RGD Reference Report - Dual and Direction-Selective Mechanisms of Phosphate Transport by the Vesicular Glutamate Transporter. - Rat Genome Database

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Dual and Direction-Selective Mechanisms of Phosphate Transport by the Vesicular Glutamate Transporter.

Authors: Preobraschenski, Julia  Cheret, Cyril  Ganzella, Marcelo  Zander, Johannes Friedrich  Richter, Karin  Schenck, Stephan  Jahn, Reinhard  Ahnert-Hilger, Gudrun 
Citation: Preobraschenski J, etal., Cell Rep. 2018 Apr 10;23(2):535-545. doi: 10.1016/j.celrep.2018.03.055.
RGD ID: 152025529
Pubmed: PMID:29642010   (View Abstract at PubMed)
DOI: DOI:10.1016/j.celrep.2018.03.055   (Journal Full-text)

Vesicular glutamate transporters (VGLUTs) fill synaptic vesicles with glutamate and are thus essential for glutamatergic neurotransmission. However, VGLUTs were originally discovered as members of a transporter subfamily specific for inorganic phosphate (Pi). It is still unclear how VGLUTs accommodate glutamate transport coupled to an electrochemical proton gradient ΔμH+ with inversely directed Pi transport coupled to the Na+ gradient and the membrane potential. Using both functional reconstitution and heterologous expression, we show that VGLUT transports glutamate and Pi using a single substrate binding site but different coupling to cation gradients. When facing the cytoplasm, both ions are transported into synaptic vesicles in a ΔμH+-dependent fashion, with glutamate preferred over Pi. When facing the extracellular space, Pi is transported in a Na+-coupled manner, with glutamate competing for binding but at lower affinity. We conclude that VGLUTs have dual functions in both vesicle transmitter loading and Pi homeostasis within glutamatergic neurons.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc17a6RatL-glutamate transmembrane transport involved_inIDA PMID:29642010UniProt 
Slc17a7RatL-glutamate transmembrane transport involved_inIDA PMID:29642010UniProt 
Slc17a6Ratphosphate ion transport involved_inIDA PMID:29642010UniProt 
Slc17a7Ratphosphate ion transport involved_inIDA PMID:29642010UniProt 
Slc17a6Ratsodium-dependent phosphate transport involved_inIDA PMID:29642010UniProt 
Slc17a7Ratsodium-dependent phosphate transport involved_inIDA PMID:29642010UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc17a7Ratsynaptic vesicle membrane located_inIDA PMID:29642010UniProt 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Slc17a6  (solute carrier family 17 member 6)
Slc17a7  (solute carrier family 17 member 7)


Additional Information