RGD Reference Report - Ion transport and regulation in a synaptic vesicle glutamate transporter. - Rat Genome Database

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Ion transport and regulation in a synaptic vesicle glutamate transporter.

Authors: Li, Fei  Eriksen, Jacob  Finer-Moore, Janet  Chang, Roger  Nguyen, Phuong  Bowen, Alisa  Myasnikov, Alexander  Yu, Zanlin  Bulkley, David  Cheng, Yifan  Edwards, Robert H  Stroud, Robert M 
Citation: Li F, etal., Science. 2020 May 22;368(6493):893-897. doi: 10.1126/science.aba9202.
RGD ID: 152025520
Pubmed: PMID:32439795   (View Abstract at PubMed)
PMCID: PMC7388591   (View Article at PubMed Central)
DOI: DOI:10.1126/science.aba9202   (Journal Full-text)

Synaptic vesicles accumulate neurotransmitters, enabling the quantal release by exocytosis that underlies synaptic transmission. Specific neurotransmitter transporters are responsible for this activity and therefore are essential for brain function. The vesicular glutamate transporters (VGLUTs) concentrate the principal excitatory neurotransmitter glutamate into synaptic vesicles, driven by membrane potential. However, the mechanism by which they do so remains poorly understood owing to a lack of structural information. We report the cryo-electron microscopy structure of rat VGLUT2 at 3.8-angstrom resolution and propose structure-based mechanisms for substrate recognition and allosteric activation by low pH and chloride. A potential permeation pathway for chloride intersects with the glutamate binding site. These results demonstrate how the activity of VGLUTs can be coordinated with large shifts in proton and chloride concentrations during the synaptic vesicle cycle to ensure normal synaptic transmission.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc17a6RatL-glutamate transmembrane transport involved_inIDA PMID:32439795UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc17a6RatL-glutamate uniporter activity enablesIDA PMID:32439795UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Slc17a6  (solute carrier family 17 member 6)


Additional Information