RGD Reference Report - CO2-sensitive preinspiratory neurons of the parafacial respiratory group express Phox2b in the neonatal rat. - Rat Genome Database

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CO2-sensitive preinspiratory neurons of the parafacial respiratory group express Phox2b in the neonatal rat.

Authors: Onimaru, Hiroshi  Ikeda, Keiko  Kawakami, Kiyoshi 
Citation: Onimaru H, etal., J Neurosci. 2008 Nov 26;28(48):12845-50. doi: 10.1523/JNEUROSCI.3625-08.2008.
RGD ID: 152025257
Pubmed: PMID:19036978   (View Abstract at PubMed)
PMCID: PMC6671793   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.3625-08.2008   (Journal Full-text)

Phox2b protein is a specific marker for neurons in the parafacial region of the ventral medulla, which are proposed to play a role in central chemoreception and postnatal survival. Mutations of PHOX2B cause congenital central hypoventilation syndrome. However, there have been no reports concerning electrophysiological characteristics of these Phox2b-expressing neurons in the parafacial region of the neonate immediately after birth. This region overlaps with the parafacial respiratory group (pFRG) composed predominantly of preinspiratory (Pre-I) neurons that are involved in respiratory rhythm generation. We studied (1) whether pFRG neurons are Phox2b immunoreactive or not and (2) whether they show intrinsic CO(2) chemosensitivity. We found that most pFRG/Pre-I neurons were Phox2b immunoreactive and depolarized upon increase in CO(2) concentration under condition of action potential-dependent synaptic transmission blockade by tetrodotoxin. We also confirmed that these pFRG neurons expressed neurokinin-1 receptor. They were tyrosine hydroxylase negative and presumed to be glutamatergic. Our findings suggest that Phox2b-expressing parafacial neurons play a role in respiratory rhythm generation as well as central chemoreception and thus are essential for postnatal survival.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to carbon dioxide  IEP 152025257 RGD 
membrane depolarization  IEP 152025257 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Phox2b  (paired-like homeobox 2b)


Additional Information