RGD Reference Report - Cafeteria Diet Consumption during Lactation in Rats, Rather than Obesity Per Se, alters miR-222, miR-200a, and miR-26a Levels in Milk. - Rat Genome Database

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Cafeteria Diet Consumption during Lactation in Rats, Rather than Obesity Per Se, alters miR-222, miR-200a, and miR-26a Levels in Milk.

Authors: Pomar, Catalina A  Castro, Heriberto  Picó, Catalina  Serra, Francisca  Palou, Andreu  Sánchez, Juana 
Citation: Pomar CA, etal., Mol Nutr Food Res. 2019 Apr;63(8):e1800928. doi: 10.1002/mnfr.201800928. Epub 2019 Feb 7.
RGD ID: 152023732
Pubmed: PMID:30698333   (View Abstract at PubMed)
DOI: DOI:10.1002/mnfr.201800928   (Journal Full-text)


SCOPE: The aim of the present study is to investigate in nursing rats the impact of cafeteria-diet feeding during lactation (cafeteria dams) on specific miRNA levels in breast milk and to discern them from the effects of maternal adiposity per se (postcafeteria dams).
METHODS AND RESULTS: Milk samples are collected from control, cafeteria, and postcafeteria dams at three time points of lactation (days 5, 10, and 15) and levels of selected miRNAs (miR-222, miR-203, miR-200a, miR-103, miR-27a, and miR-26a) were determined. Levels in milk of miR-222 rise while miR-103 and miR-27 fall throughout lactation. Moreover, at day 15 of lactation, cafeteria dams present higher miR-222 and lower miR-200a and miR-26a levels in milk than controls. No differences are found in postcafeteria dams compared with controls. At weaning, the offspring of cafeteria dams, but not the offspring of postcafeteria dams, displays lower expression levels of Cdkn1c (a validated target gene of miR-222) in liver than controls.
CONCLUSION: Cafeteria diet intake in nursing rats, rather than obesity per se, leads to alterations in specific miRNA levels, which, through the milk supply, may alter expression of target genes and potentially affect offspring phenotype.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
lactation  IEP 152023732; 152023732 RGD 
response to nutrient levels  IEP 152023732 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
extracellular space  IDA 152023732 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir222  (microRNA 222)
Mir27a  (microRNA 27a)


Additional Information