RGD Reference Report - CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma. - Rat Genome Database

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CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma.

Authors: Zhao, Bao-Cheng  Wang, Zhen-Jun  Mao, Wei-Zheng  Ma, Hua-Chong  Han, Jia-Gang  Zhao, Bo  Xu, Hui-Min 
Citation: Zhao BC, etal., World J Gastroenterol. 2011 May 21;17(19):2389-96. doi: 10.3748/wjg.v17.i19.2389.
RGD ID: 152023624
Pubmed: PMID:21633638   (View Abstract at PubMed)
PMCID: PMC3103791   (View Article at PubMed Central)
DOI: DOI:10.3748/wjg.v17.i19.2389   (Journal Full-text)


AIM: To investigate the role of CXC chemokine receptor-4 (CXCR4) and stromal cell-derived factor-1 (SDF-1) in lymph node metastasis of gastric carcinoma.
METHODS: In 40 cases of gastric cancer, expression of CXCR4 mRNA in cancer and normal mucous membrane and SDF-1 mRNA in lymph nodes around the stomach was detected using quantitative polymerase chain reaction (PCR) (TaqMan) and immunohistochemistry assay. SGC-7901 and MGC80-3 cancer cells were used to investigate the effect of SDF-1 on cell proliferation and migration.
RESULTS: Quantitative reverse transcription PCR and immunohistochemistry revealed that the expression level of CXCR4 in gastric cancer was significantly higher than that in normal mucous membrane (1.6244 ± 1.3801 vs 1.0715 ± 0.5243, P < 0.05). The expression level of CXCR4 mRNA in gastric cancer with lymph node metastasis was also significantly higher than that without lymph node metastasis (0.823 ± 0.551 vs 0.392 ± 0.338, P < 0.05). CXCR4 expression was significantly related to poorly differentiated, high tumor stage and lymph node metastasis. Significant differences in the expression level of SDF-1 mRNA were found between lymph nodes in metastatic gastric cancer and normal nodes (0.5432 ± 0.4907 vs 0.2640 ± 0.2601, P < 0.05). The positive expression of SDF-1 mRNA in lymph nodes of metastatic gastric cancer was consistent with the positive expression of CXCR4 mRNA in gastric cancer (r = 0.776, P < 0.01). Additionally, human gastric cancer cell lines expressed CXCR4 and showed vigorous proliferation and migratory responses to SDF-1. AMD3100 (a specific CXCR4 antagonist) was also found to effectively reduce the migration of gastric cancer cells.
CONCLUSION: The CXCR4/SDF-1 axis is involved in the lymph node metastasis of gastric cancer. CXCR4 is considered as a potential therapeutic target in the treatment of gastric cancer.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Lymphatic Metastasis disease_progressionIEP 152023624associated with stomach cancer and mRNA:increased expression:stomach (human)RGD 
Lymphatic Metastasis disease_progressionISOCXCL12 (Homo sapiens)152023624; 152023624associated with stomach cancer and mRNA:increased expression:stomach (human)RGD 
stomach cancer disease_progressionIEP 152023624mRNA:increased expression:stomach (human)RGD 
stomach cancer disease_progressionISOCXCR4 (Homo sapiens)152023624; 152023624mRNA:increased expression:stomach (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cxcl12  (C-X-C motif chemokine ligand 12)
Cxcr4  (C-X-C motif chemokine receptor 4)

Genes (Mus musculus)
Cxcl12  (C-X-C motif chemokine ligand 12)
Cxcr4  (C-X-C motif chemokine receptor 4)

Genes (Homo sapiens)
CXCL12  (C-X-C motif chemokine ligand 12)
CXCR4  (C-X-C motif chemokine receptor 4)


Additional Information