RGD Reference Report - Macrophage colony-stimulating factor induces prolactin expression in rat pituitary gland. - Rat Genome Database

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Macrophage colony-stimulating factor induces prolactin expression in rat pituitary gland.

Authors: Hoshino, Satoya  Kurotani, Reiko  Miyano, Yuki  Sakahara, Satoshi  Koike, Kanako  Maruyama, Minoru  Ishikawa, Fumio  Sakatai, Ichiro  Abe, Hiroyuki  Sakai, Takafumi 
Citation: Hoshino S, etal., Zoolog Sci. 2014 Jun;31(6):390-7. doi: 10.2108/zs130226.
RGD ID: 151667420
Pubmed: PMID:24882100   (View Abstract at PubMed)
DOI: DOI:10.2108/zs130226   (Journal Full-text)

We investigated the role of macrophage colony-stimulating factor (M-CSF) in the pituitary gland to understand the effect of M-CSF on pituitary hormones and the relationship between the endocrine and immune systems. When we attempted to establish pituitary cell lines from a thyrotropic pituitary tumor (TtT), a macrophage cell line, TtT/M-87, was established. We evaluated M-CSF-like activity in conditioned media (CM) from seven pituitary cell lines using TtT/M-87 cells. TtT/M-87 proliferation significantly increased in the presence of CM from TtT/GF cells, a pituitary folliculostellate (FS) cell line. M-CSF mRNA was detected in TtT/GF and MtT/E cells by reverse transcriptase-polymerase chain reaction (RT-PCR), and its expression in TtT/GF cells was increased in a lipopolysaccharide (LPS) dose-dependent manner. M-CSF mRNA expression was also increased in rat anterior pituitary glands by LPS. M-CSF receptor (M-CSFR) mRNA was only detected in TtT/ M-87 cells and increased in the LPS-stimulated rat pituitary glands. In rat pituitary glands, M-CSF and M-CSFR were found to be localized in FS cells and prolactin (PRL)-secreting cells, respectively, by immunohistochemistry. The PRL concentration in rat sera was significantly increased at 24 h after M-CSF administration, and mRNA levels significantly increased in primary culture cells of rat anterior pituitary glands. In addition, TNF-α mRNA was increased in the primary culture cells by M-CSF. These results revealed that M-CSF was secreted from FS cells and M-CSF regulated PRL expression in rat pituitary glands.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to lipopolysaccharide  IEP 151667420; 151667420 RGD 
response to macrophage colony-stimulating factor  IEP 151667420; 151667420 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
extracellular space  IDA 151667420 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Csf1  (colony stimulating factor 1)
Csf1r  (colony stimulating factor 1 receptor)
Prl  (prolactin)
Tnf  (tumor necrosis factor)


Additional Information