RGD Reference Report - Functional alterations in adult and fetal hemoglobin Sassari Asp-alpha 126(H9)-->His. The role of alpha 1 alpha 2 contact. - Rat Genome Database

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Functional alterations in adult and fetal hemoglobin Sassari Asp-alpha 126(H9)-->His. The role of alpha 1 alpha 2 contact.

Authors: Sanna, M T  Giardina, B  Scatena, R  Pellegrini, M  Olianas, A  Manca, L  Masala, B  Castagnola, M  Corda, M 
Citation: Sanna MT, etal., J Biol Chem. 1994 Jul 15;269(28):18338-42.
RGD ID: 151665732
Pubmed: (View Article at PubMed) PMID:7518430

The effects of pH, organic phosphates (2,3-diphosphoglycerate), and temperature on the functional properties of both adult and fetal hemoglobin Sassari alpha (Asp-126-->His) have been studied. The functional properties of the adult variant are characterized by the following: (i) an oxygen affinity higher than that of normal HbA in all the experimental conditions used; (ii) a dramatic reduction of homotropic interactions (n50 very close to unity); and (iii) a significant decrease of the effect of 2,3-diphosphoglycerate, which is 35% lower than that observed on HbA. The fetal variant shows an increased oxygen affinity compared with normal HbF and an almost abolished heme-heme interaction. The molecular basis of these functional differences is discussed in terms of the possible role played by the substitution of alpha (Asp-26-->His) on the stability of the R state of the molecule due to a decreased interaction at the level of alpha 1 alpha 2 contact.



Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Hba-a1  (hemoglobin alpha, adult chain 1)
Hba-a2  (hemoglobin alpha, adult chain 2)
Hbb  (hemoglobin subunit beta)
Hbb-b2  (hemoglobin, beta adult minor chain)
Hbb-bs  (hemoglobin, beta adult s chain)
LOC100134871  (beta globin minor gene)


Additional Information