RGD Reference Report - Targeting of the receptor protein tyrosine phosphatase beta with a monoclonal antibody delays tumor growth in a glioblastoma model. - Rat Genome Database

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Targeting of the receptor protein tyrosine phosphatase beta with a monoclonal antibody delays tumor growth in a glioblastoma model.

Authors: Foehr, Erik D  Lorente, Gustavo  Kuo, Jane  Ram, Rosie  Nikolich, Karoly  Urfer, Roman 
Citation: Foehr ED, etal., Cancer Res. 2006 Feb 15;66(4):2271-8. doi: 10.1158/0008-5472.CAN-05-1221.
RGD ID: 151660504
Pubmed: PMID:16489031   (View Abstract at PubMed)
DOI: DOI:10.1158/0008-5472.CAN-05-1221   (Journal Full-text)

The receptor protein tyrosine phosphatase beta (RPTPbeta) is a functional biomarker for several solid tumor types. RPTPbeta expression is largely restricted to the central nervous system and overexpressed primarily in astrocytic tumors. RPTPbeta is known to facilitate tumor cell adhesion and migration through interactions with extracellular matrix components and the growth factor pleiotrophin. Here, we show that RPTPbeta is expressed in a variety of solid tumor types with low expression in normal tissue. To assess RPTPbeta as a potential target for treatment of glioblastoma and other cancers, antibodies directed to RPTPbeta have been developed and profiled in vitro and in vivo. The recombinant extracellular domain of human short RPTPbeta was used to immunize mice and generate monoclonal antibodies that selectively recognize RPTPbeta and bind to the antigen with low nanomolar affinities. Moreover, these antibodies recognized the target on living tumor cells as measured by flow cytometry. These antibodies killed glioma cells in vitro when coupled to the cytotoxin saporin either directly or via a secondary antibody. Finally, in vivo studies showed that an anti-RPTPbeta immunotoxin (7E4B11-SAP) could significantly delay human U87 glioma tumors in a mouse xenograft model. Unconjugated 7E4B11 provides a modest but statistically significant tumor growth delay when delivered systemically in mice bearing U87 glioma tumors.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PTPRBHumanglioblastoma treatmentIMP human cells in mouse modelRGD 
PtprbRatglioblastoma treatmentISOPTPRB (Homo sapiens)human cells in mouse modelRGD 
PtprbMouseglioblastoma treatmentISOPTPRB (Homo sapiens)human cells in mouse modelRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ptprb  (protein tyrosine phosphatase, receptor type, B)

Genes (Mus musculus)
Ptprb  (protein tyrosine phosphatase receptor type B)

Genes (Homo sapiens)
PTPRB  (protein tyrosine phosphatase receptor type B)


Additional Information