RGD Reference Report - L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier. - Rat Genome Database

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L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier.

Authors: Tomi, Masatoshi  Mori, Masahiko  Tachikawa, Masanori  Katayama, Kazunori  Terasaki, Tetsuya  Hosoya, Ken-ichi 
Citation: Tomi M, etal., Invest Ophthalmol Vis Sci. 2005 Jul;46(7):2522-30. doi: 10.1167/iovs.04-1175.
RGD ID: 151361128
Pubmed: PMID:15980244   (View Abstract at PubMed)
DOI: DOI:10.1167/iovs.04-1175   (Journal Full-text)


PURPOSE: L-type amino acid transporters (LATs) prefer branched-chain and aromatic amino acids, including neurotransmitter precursors. The objective of this study was to clarify the expression and function of LAT at the inner blood-retinal barrier (BRB).
METHODS: [3H]L-Leucine transport at the inner BRB was characterized by using in vivo integration plot analysis and a conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB2). The expression of the LAT1 was demonstrated by quantitative real-time RT-PCR, immunoblot, and immunohistochemical analyses.
RESULTS: The apparent influx permeability clearance of [3H]L-leucine in the rat retina was found to be 203 microL/(min.g retina), supporting a carrier-mediated influx transport of L-leucine at the BRB. [3H]L-Leucine uptake by TR-iBRB2 cells was an Na+-independent and concentration-dependent process with a Km of 14.1 microM. This process was more potently cis inhibited by substrates of LAT1, D-leucine, D-phenylalanine, and D-methionine, than those of LAT2, L-alanine, and L-glutamine. [3H]L-Leucine efflux from TR-iBRB2 cells was trans-stimulated by substrates of LAT1. The expression of LAT1 mRNA was 100- and 15-fold greater than that of LAT2 in TR-iBRB2 and magnetically isolated rat retinal vascular endothelial cells, respectively. The expression of LAT1 protein was observed in TR-iBRB2 and primary cultured human retinal endothelial cells and immunostaining of LAT1 was observed along the rat retinal capillaries.
CONCLUSIONS: LAT1 is expressed at the inner BRB and mediates blood-to-retina L-leucine transport. This transport system plays a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
L-amino acid transport  IMP 151361128 RGD 
L-leucine transport involved_inIDA 151361128PMID:15980244UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Slc7a5  (solute carrier family 7 member 5)


Additional Information