RGD Reference Report - Abundance and Significance of Neuroligin-1 and Neurexin II in the Enteric Nervous System of Embryonic Rats. - Rat Genome Database

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Abundance and Significance of Neuroligin-1 and Neurexin II in the Enteric Nervous System of Embryonic Rats.

Authors: Wang, Dongming  Pan, Jingnian  Song, Guoxin  Gao, Ni  Zheng, Yi  Zhang, Qiangye  Li, Aiwu 
Citation: Wang D, etal., Biomed Res Int. 2017;2017:1209360. doi: 10.1155/2017/1209360. Epub 2017 Jan 18.
RGD ID: 151356632
Pubmed: PMID:28194405   (View Abstract at PubMed)
PMCID: PMC5286486   (View Article at PubMed Central)
DOI: DOI:10.1155/2017/1209360   (Journal Full-text)

Aim. To investigate the abundance of neuroligin-1 and neurexin II in the enteric nervous system (ENS) of rats on different embryonic days and to explore their potential significance. Methods. The full-thickness colon specimens proximal to the ileocecal junction of rats on embryonic days 16, 18, and 20 and of newborns within 24 hours (E16, E18, E20, and Ep0) were studied, respectively. qRT-PCR was applied for detecting the expressions of neuroligin-1 and neurexin II on mRNA, and western blotting was employed for detecting their further expressions on the whole tissue. Finally, the histological appearance of neuroligin-1 and neurexin IIα was elucidated using immunohistochemical staining. Results. qRT-PCR showed that the neuroligin-1 and neurexin II mRNA expressions of groups E16, E18, E20, and Ep0 increased gradually with the growth of embryonic rats (P < 0.05). Western blotting confirmed the increasing tendency. In immunohistochemical staining, proteins neuroligin-1 and neurexin IIα positive cells concentrated mostly in the myenteric nerve plexus of the colon and their expressions depend on the embryonic time. Conclusion. Neuroligin-1 and neurexin II were both expressed in the ENS and have temporal correlation with the development of ENS, during which neuronal intestinal malformations (NIM) may occur due to their disruptions and consequent abnormal ENS development.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nlgn1Ratpositive regulation of neuromuscular synaptic transmission involved_inNAS PMID:28194405ComplexPortal 
Nrxn1Ratpositive regulation of neuromuscular synaptic transmission involved_inNAS PMID:28194405ComplexPortal 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nlgn1Ratneuromuscular junction part_ofNAS PMID:28194405ComplexPortal 
Nrxn1Ratneuromuscular junction part_ofNAS PMID:28194405ComplexPortal 

Objects Annotated

Genes (Rattus norvegicus)
Nlgn1  (neuroligin 1)
Nrxn1  (neurexin 1)


Additional Information