RGD Reference Report - Bidirectional substrate fluxes through the system N (SNAT5) glutamine transporter may determine net glutamine flux in rat liver. - Rat Genome Database

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Bidirectional substrate fluxes through the system N (SNAT5) glutamine transporter may determine net glutamine flux in rat liver.

Authors: Baird, F E  Beattie, K J  Hyde, A R  Ganapathy, V  Rennie, M J  Taylor, P M 
Citation: Baird FE, etal., J Physiol. 2004 Sep 1;559(Pt 2):367-81. doi: 10.1113/jphysiol.2003.060293. Epub 2004 Jun 24.
RGD ID: 15090853
Pubmed: PMID:15218073   (View Abstract at PubMed)
PMCID: PMC1665133   (View Article at PubMed Central)
DOI: DOI:10.1113/jphysiol.2003.060293   (Journal Full-text)

System N (SNAT3 and SNAT5) amino acid transporters are key mediators of glutamine transport across the plasma membrane of mammalian cell types, including hepatocytes and astrocytes. We demonstrate that SNAT5 shows simultaneous bidirectional glutamine fluxes when overexpressed in Xenopus oocytes. Influx and efflux are both apparently Na+ dependent but, since they are not directly coupled, the carrier is capable of mediating net amino acid movement across the cell membrane. The apparent Km values for glutamine influx and efflux are similar (approximately 1 mm) and the transporter behaviour is consistent with a kinetic model in which re-orientation of the carrier from outside- to inside-facing conformations (either empty or substrate loaded) is the limiting step in the transport cycle. In perfused rat liver, the observed relationship between influent (portal) glutamine concentration and net hepatic glutamine flux may be described by a simple kinetic model, assuming the balance between influx and efflux through System N determines net flux, where under physiological conditions efflux is generally saturated owing to high intracellular glutamine concentration. SNAT5 shows a more periportal mRNA distribution than SNAT3 in rat liver, indicating that SNAT5 may have particular importance for modulation of net hepatic glutamine flux.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc38a5Ratamino acid export across plasma membrane involved_inIDA PMID:15218073UniProt 
Slc38a5Ratamino acid import across plasma membrane involved_inIDA PMID:15218073UniProt 
Slc38a2Ratglutamine transport involved_inIDA PMID:15218073ARUK-UCL 
Slc38a5Ratglutamine transport involved_inIDA PMID:15218073ARUK-UCL 
Slc38a5RatL-glutamine import across plasma membrane involved_inIDA PMID:15218073UniProt 
Slc38a5RatL-histidine transmembrane transport involved_inIDA PMID:15218073ARUK-UCL 
Slc38a2RatL-serine transport involved_inIDA PMID:15218073ARUK-UCL 
Slc38a5RatL-serine transport involved_inIDA PMID:15218073ARUK-UCL 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Slc38a2  (solute carrier family 38, member 2)
Slc38a5  (solute carrier family 38, member 5)


Additional Information