RGD Reference Report - Protein expression, characterization, and regulation of CYP4F4 and CYP4F5 cloned from rat brain. - Rat Genome Database

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Protein expression, characterization, and regulation of CYP4F4 and CYP4F5 cloned from rat brain.

Authors: Kawashima, H  Kusunose, E  Thompson, C M  Strobel, H W 
Citation: Kawashima H, etal., Arch Biochem Biophys. 1997 Nov 1;347(1):148-54. doi: 10.1006/abbi.1997.0342.
RGD ID: 15090848
Pubmed: PMID:9344476   (View Abstract at PubMed)
DOI: DOI:10.1006/abbi.1997.0342   (Journal Full-text)

We previously reported the cDNA cloning of three new forms of P450, CYP4F4, CYP4F5, and CYP4F6, from a rat brain cDNA library. In the present study, we expressed CYP4F4 and CYP4F5 in Escherichia coli using the pCWOri expression vector with a modification of their N-terminal amino acid sequences and the incorporation of a C-terminal [His]4 tag to aid in purification. CYP4F5 recombinant protein was purified to a specific content of 7.7 nmol/mg protein from the membrane fraction of E. coli and showed omega-hydroxylation activity toward leukotriene B4 (LTB4), a chemical mediator of inflammation. On the other hand, the solubilized membrane fraction of CYP4F4-expressed recombinant protein catalyzed the omega-hydroxylation of prostaglandin A1, prostaglandin E1, and 6-trans-LTB4 as well as LTB4. The effects of the peroxisome proliferator, clofibrate, on mRNA expression of CYP4F4, 4F5, and 4F6 were studied by Northern blot analysis. The expression levels of the mRNA of these CYP4Fs were shown to be reduced by clofibrate in liver.



Gene Ontology Annotations    

Biological Process

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Cyp4f4  (cytochrome P450, family 4, subfamily f, polypeptide 4)


Additional Information