RGD Reference Report - Expression of proto-oncogene cFMS protein in lung, breast, and ovarian cancers. - Rat Genome Database

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Expression of proto-oncogene cFMS protein in lung, breast, and ovarian cancers.

Authors: Kakiuchi-Kiyota, Satoko  Lappin, Patrick B  Heintz, Catherine  Brown, Paul W  Pinho, Flavia O  Ryan, Anne M  Mathialagan, Nagappan 
Citation: Kakiuchi-Kiyota S, etal., Appl Immunohistochem Mol Morphol. 2014 Mar;22(3):188-99. doi: 10.1097/PAI.0b013e31828e7104.
RGD ID: 150524280
Pubmed: PMID:23702648   (View Abstract at PubMed)
DOI: DOI:10.1097/PAI.0b013e31828e7104   (Journal Full-text)

We performed immunohistochemistry for macrophage colony-stimulating factor 1 receptor (also known as c-fms proto-oncogene product) on tissue microarrays of human nontumor lung, pulmonary squamous cell carcinomas (SCC) and adenocarcinomas (ADC), and breast and ovarian carcinomas using a commercially available anti-cFMS antibody. The specificity of the antibody was validated by Western blot and mass spectrometry analysis. Staining of cFMS was restricted to stromal fibroblasts in pulmonary SCC and ADC specimens and was not identified in tumor epithelium or epithelium and stromal cells of nontumor lung. Evaluation of pulmonary SCC (n=63) and ADC (n=71) specimens revealed stromal fibroblast cFMS staining in 60% (38 of 63) and 35% (25 of 71) of the tumor samples, respectively. A similar pattern of stromal fibroblast cFMS staining was observed in breast (n=21) and ovarian (n=50) carcinomas. It was reported that glucocorticoids induced cFMS expression in breast carcinomas and choriocarcinomas. To investigate whether stromal cFMS expression in lung cancers was associated with glucocorticoid signaling, glucocorticoid receptor protein distribution was evaluated in lung tissue microarrays by immunohistochemistry. Stromal fibroblast glucocorticoid receptor staining was only observed in 18% (2 of 11) of pulmonary SCC and 6% (1 of 17) of ADC specimens, suggesting that cFMS expression may not be directly mediated by glucocorticoids in stromal fibroblasts of lung cancers. The tumor stromal cell expression of cFMS in certain tumor types (lung, ovarian, and breast) suggests the potential for more diverse tumor therapeutic options and presents an attractive target for drug development.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
lung adenocarcinoma  IEP 150524280protein:increased expression:lung (human)RGD 
lung adenocarcinoma  ISOCSF1R (Homo sapiens)150524280; 150524280protein:increased expression:lung (human)RGD 
lung squamous cell carcinoma  IEP 150524280protein:increased expression:lung (human)RGD 
lung squamous cell carcinoma  ISOCSF1R (Homo sapiens)150524280; 150524280protein:increased expression:lung (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Csf1r  (colony stimulating factor 1 receptor)

Genes (Mus musculus)
Csf1r  (colony stimulating factor 1 receptor)

Genes (Homo sapiens)
CSF1R  (colony stimulating factor 1 receptor)


Additional Information