RGD Reference Report - Combination of CALR and PDIA3 is a potential prognostic biomarker for non-small cell lung cancer. - Rat Genome Database

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Combination of CALR and PDIA3 is a potential prognostic biomarker for non-small cell lung cancer.

Authors: Wang, Ke  Li, Hao  Chen, Ruo  Zhang, Yang  Sun, Xiu-Xuan  Huang, Wan  Bian, Huijie  Chen, Zhi-Nan 
Citation: Wang K, etal., Oncotarget. 2017 Jun 16;8(57):96945-96957. doi: 10.18632/oncotarget.18547. eCollection 2017 Nov 14.
RGD ID: 150521696
Pubmed: PMID:29228584   (View Abstract at PubMed)
PMCID: PMC5722536   (View Article at PubMed Central)
DOI: DOI:10.18632/oncotarget.18547   (Journal Full-text)

Proteomic-based approaches for biomarker discovery are promising strategies used in cancer research. In this study, we performed quantitative proteomic analysis on 16 paired samples of non-small cell lung cancer (NSCLC) and adjacent non-tumor lung tissues using label-free quantitative proteomics and liquid chromatography-tandem mass spectrometry/mass spectrometry (LC-MS/MS) to identify differentially expressed proteins. A total of 91 proteins were differentially expressed in NSCLC compared with adjacent non-tumor lung tissues among 4047 identified proteins (fold change > 1.5 or < 0.67, P < 0.05). Gene ontology (GO) analysis, Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis and ingenuity pathway analysis (IPA) of 91 dysregulated proteins showed that they were related to the cancer-associated biological processes. We confirmed that the candidate proteins, calreticulin (CALR) and protein disulfide isomerase family A member 3 (PDIA3) were overexpressed in NSCLC by real-time PCR using 20 paired samples and western blot using 5 paired samples. PDIA3 expression was highly associated with CALR expression (Spearman r = 0.345, P = 0.001) and they were co-localized and interacted with each other in A549 and H460 cells. Moreover, survival analysis performed in tissue microarray with 88 samples indicated that low expression of both CALR and PDIA3 in NSCLC was positively associated with poor overall survival. Combination of CALR and PDIA3 might serve as an efficient biomarker and improved the prediction of NSCLC prognosis significantly (P = 0.023). Our results collectively provide a potential biomarker dataset for NSCLC prognosis, especially the prognostic value of combined expression of CALR and PDIA3.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
lung non-small cell carcinoma amelioratesIEP 150521696mRNA and protein:increased expression:lung (human)RGD 
lung non-small cell carcinoma amelioratesISOCALR (Homo sapiens)150521696; 150521696mRNA and protein:increased expression:lung (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Calr  (calreticulin)

Genes (Mus musculus)
Calr  (calreticulin)

Genes (Homo sapiens)
CALR  (calreticulin)


Additional Information