RGD Reference Report - Effect of Bushenhuoxue formula on interleukin-1 beta and discoidin domain receptor 2 levels in a rat model of osteoarthritis. - Rat Genome Database

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Effect of Bushenhuoxue formula on interleukin-1 beta and discoidin domain receptor 2 levels in a rat model of osteoarthritis.

Authors: Nianhu, Li  Lei, Xia  Sheng, Yi  Jianhui, Yu  Yi, Zhai  Zhanwang, Xu 
Citation: Nianhu L, etal., J Tradit Chin Med. 2015 Apr;35(2):192-6. doi: 10.1016/s0254-6272(15)30027-3.
RGD ID: 150429975
Pubmed: PMID:25975052   (View Abstract at PubMed)
DOI: DOI:10.1016/s0254-6272(15)30027-3   (Journal Full-text)


OBJECTIVE: To determine the effects of Bushenhuoxue formula (BHF) on interleukin-1 beta (IL-1β), transforming growth factor beta 1 (TGF-β1), discoidin domain receptor 2 (DDR2) and matrix metalloproteinase-1 (MMP-1) levels in a rat model of osteoarthritis (OA).
METHODS: Sprague-Dawley rats were used to establish an OA model and subjected to various treatments over 6 weeks. Rats were treated with BHF, glucosamine sulfate (GS), or starch as a control. Serum levels of IL-1β and MMP-1 and joint fluid levels of IL-1β were determined by means of ELISAs. We used immunohistochemistry to determine DDR2 levels in knee cartilage. Gene expression levels of MMP-1 in joint synovial tissue were assessed using reverse transcription polymerase chain reaction assays.
RESULTS: Serum IL-1β levels were unchanged throughout the study. Levels of IL-1β in joint fluid and MMP-1 in sera from the BHF- and GS-treated groups were significantly reduced. DDR2 levels in knee cartilage were also significantly reduced in the BHF group. Expression of the MMP-1 gene was significantly reduced by BHF treatment.
CONCLUSION: BHF might be beneficial in the inhibition and alleviation of local inflammatory responses and cartilage degeneration in OA.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Osteoarthritis, Experimental treatmentISODdr2 (Rattus norvegicus)150429975; 150429975 RGD 
Osteoarthritis, Experimental treatmentIEP 150429975 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ddr2  (discoidin domain receptor tyrosine kinase 2)

Genes (Mus musculus)
Ddr2  (discoidin domain receptor family, member 2)

Genes (Homo sapiens)
DDR2  (discoidin domain receptor tyrosine kinase 2)


Additional Information