RGD Reference Report - Resveratrol attenuates excessive ethanol exposure induced insulin resistance in rats via improving NAD+ /NADH ratio. - Rat Genome Database

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Resveratrol attenuates excessive ethanol exposure induced insulin resistance in rats via improving NAD+ /NADH ratio.

Authors: Luo, Gang  Huang, Bingqing  Qiu, Xiang  Xiao, Lin  Wang, Ning  Gao, Qin  Yang, Wei  Hao, Liping 
Citation: Luo G, etal., Mol Nutr Food Res. 2017 Nov;61(11). doi: 10.1002/mnfr.201700087. Epub 2017 Aug 15.
RGD ID: 14981582
Pubmed: PMID:28688179   (View Abstract at PubMed)
DOI: DOI:10.1002/mnfr.201700087   (Journal Full-text)


SCOPE: Resveratrol has been shown to improve insulin resistance via activating the NAD+ -dependent deacetylase SIRT1, but the effects of resveratrol on ethanol-induced insulin resistance remain unclear. This study was designed to explore the potential mechanism by which resveratrol ameliorated ethanol-induced insulin resistance, focusing on its regulations on the ratio of NAD+ /NADH and SIRT1 expression.
METHODS AND RESULTS: Male Sprague-Dawley rats were fed either control or ethanol liquid diets containing 0.8, 1.6 and 2.4 g/kg·bw ethanol with or without 100 mg/kg·bw resveratrol for 22 weeks. Resveratrol improved ethanol (2.4 g/kg·bw) induced reductions in insulin sensitivity, SIRT1 expression (51%, P < 0.05), NAD+ /NADH ratio (196%, P < 0.01) as well as the expression and activity of ALDH2 while decreased the augmentations in the expression and activity of ADH and CYP2E1. In primary rat hepatocytes, ethanol exposure (25 mmol/L, 24 h) similarly decreased SIRT1 expression and NAD+ /NADH ratio (33%, P < 0.05; 32%, P < 0.01), and 0.1 μmol/L resveratrol treatment reversed these decreases and inhibited the expressions of ADH and CYP2E1.
CONCLUSION: Resveratrol exhibits benefits against ethanol-induced insulin resistance via improving the ratio of NAD+ /NADH to regulate SIRT1, which is associated with the modulation of ethanol metabolism enzymes.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Insulin Resistance treatmentISOAldh2 (Rattus norvegicus)14981582; 14981582protein:altered expression and altered activity:liver (rat)RGD 
Insulin Resistance treatmentIEP 14981582protein:altered expression and altered activity:liver (rat)RGD 

Gene-Chemical Interaction Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ethanol decreases expressionISOAldh2 (Rattus norvegicus)14981582; 14981582ethanol decreases expression and activity of Aldh2 protein in rat liverRGD 
ethanol decreases expressionEXP 14981582ethanol decreases expression and activity of Aldh2 protein in rat liverRGD 
resveratrol increases expressionISOAldh2 (Rattus norvegicus)14981582; 14981582Resveratrol increases expression and activity of Aldh2 protein in rat liverRGD 
resveratrol increases expressionEXP 14981582Resveratrol increases expression and activity of Aldh2 protein in rat liverRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to resveratrol  IEP 14981582protein:altered expression and altered activity:liver ratRGD 
ethanol metabolic process  IEP 14981582protein:altered expression and altered activity:liver ratRGD 
response to ethanol  IEP 14981582protein:altered expression and altered activity:liver ratRGD 

Objects Annotated

Genes (Rattus norvegicus)
Aldh2  (aldehyde dehydrogenase 2 family member)

Genes (Mus musculus)
Aldh2  (aldehyde dehydrogenase 2, mitochondrial)

Genes (Homo sapiens)
ALDH2  (aldehyde dehydrogenase 2 family member)


Additional Information