RGD Reference Report - Molecular analysis of interleukin-10 gene polymorphisms in patients with Behçet's disease. - Rat Genome Database

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Molecular analysis of interleukin-10 gene polymorphisms in patients with Behçet's disease.

Authors: Afkari, Babak  Babaloo, Zohreh  Dolati, Sanam  Khabazi, Alireza  Jadidi-Niaragh, Farhad  Talei, Mahsa  Shanehbandi, Dariush  Mahmoudi, Shiva  Hazhirkarzar, Bita  Sakhinia, Ebrahim 
Citation: Afkari B, etal., Immunol Lett. 2018 Feb;194:56-61. doi: 10.1016/j.imlet.2017.12.008. Epub 2017 Dec 30.
RGD ID: 14975131
Pubmed: (View Article at PubMed) PMID:29294320
DOI: Full-text: DOI:10.1016/j.imlet.2017.12.008


BACKGROUND: Interleukin 10 (IL-10) is a cytokine with potent anti-inflammatory properties that play a fundamental role in restrictive host immune response to pathogens, by means of that is a crucial importance for chronic inflammatory disease studies. Therefore, the goal of this study was to measure the correlation of the IL-10 gene polymorphisms with the susceptibility to Behçet's disease compared with the control group in the Azeri population and to determine the expression of this gene in the two groups. Also, real-time PCR was performed for evaluate the IL-10 mRNA expression of the associated polymorphisms.
METHODS: In this study, blood samples from 47 (1 missed) patients and 58 (3 missed) healthy control were taken, and then mononuclear cells isolated with ficoll protocol. The DNA and RNA were subsequently extracted. They were examined for -592A/C (rs1800872) of IL-10 gene single nucleotide polymorphism (SNP) using RFLP-PCR. Allele and genotype distributions were evaluated among groups using chi-square or Fisher's test. Following this, the extracted RNA was converted to cDNA using the RT-PCR method, after that expression of IL-10 evaluated by Real-time PCR. Serum levels of IL-10 were measured using Enzyme-linked immunosorbent assay (ELISA).
RESULTS: Rates of the rs1800872 A allele was statistically lower in the control group compared with BD patients (p = 0.0315 and OR = 1.90 (1.05-3.42)). Also, as we expected, the expression level of the IL-10 gene was seen to significantly decrease in the patient group compared to the control.
CONCLUSIONS: Our study showed that the rs1800872 A allele of the IL-10 gene may contribute to the genetic susceptibility of BD by regulating the expression of IL-10. Also as we expected, the expression level of this gene was seen to significantly decrease in the patient group compared to the control.

Annotation

Disease Annotations    
Behcet's disease  (IAGP,ISO)

Objects Annotated

Genes (Rattus norvegicus)
Il10  (interleukin 10)

Genes (Mus musculus)
Il10  (interleukin 10)

Genes (Homo sapiens)
IL10  (interleukin 10)


Additional Information