RGD Reference Report - Beta-cell specific deletion of Dicer1 leads to defective insulin secretion and diabetes mellitus. - Rat Genome Database

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Beta-cell specific deletion of Dicer1 leads to defective insulin secretion and diabetes mellitus.

Authors: Kalis, Martins  Bolmeson, Caroline  Esguerra, Jonathan L S  Gupta, Shashank  Edlund, Anna  Tormo-Badia, Neivis  Speidel, Dina  Holmberg, Dan  Mayans, Sofia  Khoo, Nelson K S  Wendt, Anna  Eliasson, Lena  Cilio, Corrado M 
Citation: Kalis M, etal., PLoS One. 2011;6(12):e29166. doi: 10.1371/journal.pone.0029166. Epub 2011 Dec 27.
RGD ID: 149735536
Pubmed: PMID:22216196   (View Abstract at PubMed)
PMCID: PMC3246465   (View Article at PubMed Central)
DOI: DOI:10.1371/journal.pone.0029166   (Journal Full-text)

Mature microRNAs (miRNAs), derived through cleavage of pre-miRNAs by the Dicer1 enzyme, regulate protein expression in many cell-types including cells in the pancreatic islets of Langerhans. To investigate the importance of miRNAs in mouse insulin secreting β-cells, we have generated mice with a β-cells specific disruption of the Dicer1 gene using the Cre-lox system controlled by the rat insulin promoter (RIP). In contrast to their normoglycaemic control littermates (RIP-Cre(+/-) Dicer1(Δ/wt)), RIP-Cre(+/-)Dicer1(flox/flox) mice (RIP-Cre Dicer1(Δ/Δ)) developed progressive hyperglycaemia and full-blown diabetes mellitus in adulthood that recapitulated the natural history of the spontaneous disease in mice. Reduced insulin gene expression and concomitant reduced insulin secretion preceded the hyperglycaemic state and diabetes development. Immunohistochemical, flow cytometric and ultrastructural analyses revealed altered islet morphology, marked decreased β-cell mass, reduced numbers of granules within the β-cells and reduced granule docking in adult RIP-Cre Dicer1(Δ/Δ) mice. β-cell specific Dicer1 deletion did not appear to disrupt fetal and neonatal β-cell development as 2-week old RIP-Cre Dicer1(Δ/Δ) mice showed ultrastructurally normal β-cells and intact insulin secretion. In conclusion, we have demonstrated that a β-cell specific disruption of the miRNAs network, although allowing for apparently normal β-cell development, leads to progressive impairment of insulin secretion, glucose homeostasis and diabetes development.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
diabetes mellitus  ISODicer1 (Mus musculus)149735536; 149735536 RGD 
diabetes mellitus  IMP 149735536 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dicer1  (dicer 1 ribonuclease III)

Genes (Mus musculus)
Dicer1  (dicer 1, ribonuclease type III)

Genes (Homo sapiens)
DICER1  (dicer 1, ribonuclease III)


Additional Information