RGD Reference Report - SAMHD1 inhibits epithelial cell transformation in vitro and affects leukemia development in xenograft mice. - Rat Genome Database

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SAMHD1 inhibits epithelial cell transformation in vitro and affects leukemia development in xenograft mice.

Authors: Kodigepalli, Karthik M  Li, Minghua  Bonifati, Serena  Panfil, Amanda R  Green, Patrick L  Liu, Shan-Lu  Wu, Li 
Citation: Kodigepalli KM, etal., Cell Cycle. 2018;17(23):2564-2576. doi: 10.1080/15384101.2018.1550955. Epub 2018 Dec 4.
RGD ID: 149735523
Pubmed: PMID:30474474   (View Abstract at PubMed)
PMCID: PMC6300106   (View Article at PubMed Central)
DOI: DOI:10.1080/15384101.2018.1550955   (Journal Full-text)

Sterile alpha motif and HD domain-containing protein 1 (SAMHD1) is a mammalian dNTP hydrolase (dNTPase) and functions as a negative regulator in the efficacy of cytarabine treatment of acute myeloid leukemia (AML). We have reported that SAMHD1 knockout (KO) increased the activity of phosphoinositide 3-kinase (PI3K) in AML-derived THP-1 cells and attenuated their ability to form subcutaneous tumors in xenografted immunodeficient mice. However, the functional significance of SAMHD1 in controlling AML leukemogenesis remains unclear. Previous studies show that in vitro transformation of Madin-Darby canine kidney (MDCK) epithelial cells by the Jaagsiekte sheep retrovirus (JSRV) envelope protein requires activation of the PI3K/Akt oncogenic signaling pathway. Using this cell transformation model, we demonstrated that ectopic expression of wild-type human SAMHD1 or a dNTPase-defective SAMHD1 mutant (HD/AA) significantly inhibited MDCK cell transformation, but did not affect cell proliferation. To visualize and quantify THP-1 cell growth and metastasis in xenografted immunodeficient mice, we generated luciferase-expressing stable SAMHD1 KO THP-1 cells and control THP-1 cells, which were injected intravenously into immunodeficient mice. Bioluminescence imaging and quantification analysis of xenografted mice revealed that SAMHD1 KO cell-derived tumors had similar growth and metastatic potential compared with control cells at 35 days post-injection. However, mice xenografted with SAMHD1 KO cells showed greater survival compared with mice injected with control cells. Our data suggest that exogenous SAMHD1 expression suppresses in vitro cell transformation independently of its dNTPase activity, and that endogenous SAMHD1 affects AML tumorigenicity and disease progression in vivo.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SAMHD1Humanacute myeloid leukemia severityIMP human cell line in mouse modelRGD 
Samhd1Ratacute myeloid leukemia severityISOSAMHD1 (Homo sapiens)human cell line in mouse modelRGD 
Samhd1Mouseacute myeloid leukemia severityISOSAMHD1 (Homo sapiens)human cell line in mouse modelRGD 

Objects Annotated

Genes (Rattus norvegicus)
Samhd1  (SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1)

Genes (Mus musculus)
Samhd1  (SAM domain and HD domain, 1)

Genes (Homo sapiens)
SAMHD1  (SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1)


Additional Information